1.5 Angstrom Crystal Structure of the Complex of Ligand Binding Component of ABC-type Import System from Staphylococcus aureus with Nickel and two Histidines

Center for Structural Genomics of Infectious Diseases (CSGID)

4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

(MSE)GSSHHHHHHENLYFQGHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTT
LDIELKDNIKFQNGQKLTAEKVKSSLENS(MSE)KKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPF(MSE)
AIYDTDAKSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNK
VQDIENNQNLKVSKESGFRTSLL(MSE)YNHTNKK(MSE)TKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYI
KEPKLTKQNIEQAK(MSE)LLAKDGYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSA
WDAT(MSE)YSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYI
AYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER

MGSSHHHHHHENLYFQGHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIE
LKDNIKFQNGQKLTAEKVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDAKSDVN
QTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVQDIENNQNLKV
SKESGFRTSLLMYNHTNKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKD
GYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYFFNQAY
KKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDY
RTTIER

Protein: 7.5mG/mL, 0.5M Sodium chloride, 0.01M Tris, pH 8.3. Screen: Classics II (D8), 0.1M HEPES, pH 7.5, 25% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K

Center for Structural Genomics of Infectious Diseases

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Ligand Binding Component, ABC-type Import System, Nickel, single or di-peptides, Structural Genomics, Center for Structural Genomics of Infectious Diseases, CSGID, UNKNOWN FUNCTION

HSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDAKSDVNQTPVGTGPYQIKDYKQS
RKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTN
KKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLKIKLITYDG
RPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSNVD
DLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER