Crystal structure of alpha-neoagarobiose hydrolase (ALPHA-NABH) in complex with alpha-d-galactopyranose from Saccharophagus degradans 2-40

alpha-D-galactose; D-galactose; galactose; ALPHA D-GALACTOSE

Crystal structure of a key enzyme in the agarolytic pathway, alpha-neoagarobiose hydrolase from Saccharophagus degradans 2-40

Glycosyl hydrolase family 32, N terminal

MSDSKVNKKLSKASLRAIERGYDEKGPEWLFEFDITPLKGDLAYEEGVIRRDPSAVLKVDDEYHVWYTKGEGETVGFGSD
NPEDKVFPWDKTEVWHATSKDKITWKEIGPAIQRGAAGAYDDRAVFTPEVLRHNGTYYLVYQTVKAPYLNRSLEHIAIAY
SDSPFGPWTKSDAPILSPENDGVWDTDEDNRFLVKEKGSFDSHKVHDPCLMFFNNRFYLYYKGETMGESMNMGGREIKHG
VAIADSPLGPYTKSEYNPITNSGHEVAVWPYKGGMATMLTTDGPEKNTCQWAEDGINFDIMSHIKGAPEAVGFFRPESDS
DDPISGIEWGLSHKYDASWNWNYLCFFKTRRQVLDAGSYQQTGDSGAVHHHHHH

2.4M SODIUM MALONATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K

AGAR METABOLISM, NEOAGAROBIOSE, 3, 6-ANHYDRO-L-GALACTOSE, ALPHA-D-GALACTOPYRANOSE, BIOENERGY, HYDROLASE

MSDSKVNKKLSKASLRAIERGYDEKGPEWLFEFDITPLKGDLAYEEGVIRRDPSAVLKVDDEYHVWYTKGEGETVGFGSD
NPEDKVFPWDKTEVWHATSKDKITWKEIGPAIQRGAAGAYDDRAVFTPEVLRHNGTYYLVYQTVKAPYLNRSLEHIAIAY
SDSPFGPWTKSDAPILSPENDGVWDTDEDNRFLVKEKGSFDSHKVHDPCLMFFNNRFYLYYKGETMGESMNMGGREIKHG
VAIADSPLGPYTKSEYNPITNSGHEVAVWPYKGGMATMLTTDGPEKNTCQWAEDGINFDIMSHIKGAPEAVGFFRPESDS
DDPISGIEWGLSHKYDASWNWNYLCFFKTRRQVLDAGSYQQTGDSGAV