Crystal structures of the murine class I major histocompatibility complex H-2Db in complex with LCMV-derived gp33 altered peptide ligand (Y4S)

Unexpected T-cell recognition of an altered peptide ligand is driven by reversed thermodynamics.

H-2 class I histocompatibility antigen, D-B alpha chain

GPHSMRYFETAVSRPGLEEPRYISVGYVDNKEFVRFDSDAENPRYEPRAPWMEQEGPEYWERETQKAKGQEQWFRVSLRN
LLGYYNQSAGGSHTLQQMSGCDLGSDWRLLRGYLQFAYEGRDYIALNEDLKTWTAADMAAQITRRKWEQSGAAEHYKAYL
EGECVEWLHRYLKNGNATLLRTDSPKAHVTHHPRSKGEVTLRCWALGFYPADITLTWQLNGEELTQDMELVETRPAGDGT
FQKWASVVVPLGKEQNYTCRVYHEGLPEPLTLRWEP

IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYAC
RVKHDSMAEPKTVYWDRDM

1.8 M ammonium sulfate, 0.1 M Tris HCl pH 9.0., VAPOR DIFFUSION, HANGING DROP, temperature 293K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Murine MHC, LCMV, receptor binding, beta2-microglobulin, immune system, T cell recognition, T cell receptor, cell surface

IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYAC
RVKHASMAEPKTVYWDRDM

BINDING SITE FOR CHAIN A OF H-2 CLASS I HISTOCOMPATIBILITY ANTIGEN, D-B ALPHA CHAIN

BINDING SITE FOR CHAIN D OF H-2 CLASS I HISTOCOMPATIBILITY ANTIGEN, D-B ALPHA CHAIN

BINDING SITE FOR CHAIN G OF H-2 CLASS I HISTOCOMPATIBILITY ANTIGEN, D-B ALPHA CHAIN

BINDING SITE FOR CHAIN J OF H-2 CLASS I HISTOCOMPATIBILITY ANTIGEN, D-B ALPHA CHAIN