Crystal structure of the phosphonate binding protein, PhnD, from Escherichia coli

Structure of the Escherichia coli Phosphonate Binding Protein PhnD and Rationally Optimized Phosphonate Biosensors.

PhnD, subunit of alkylphosphonate ABC transporter

(MSE)HHHHHHGSEEQEKALNFGIISTESQQNLKPQWTPFLQD(MSE)EKKLGVKVNAFFAPDYAGIIQG(MSE)RFNKV
DIAWYGNLSA(MSE)EAVDRANGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVPG
YYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKELKVIWKSPLIPGDPIVWRKNLSE
TTKDKIYDFF(MSE)NYGKTPEEKAVLERLGWAPFRASSDLQLVPIRQLALFKE(MSE)QSVKDNKGLNEQDKLAKTTAI
QAQLDDLDRLNNALSA(MSE)SSVSKAVQ

MHHHHHHGSEEQEKALNFGIISTESQQNLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAME
AVDRANGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVPGYYVFAKNNISASDFKR
TVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKELKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKT
PEEKAVLERLGWAPFRASSDLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVSKAV
Q

0.2M Magnesium chloride hexahydrate, 0.1M Bis-tris, 25%(w/v) PEG 3,350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

The same protein, but different space group. Both structures show the presence of an unknown ligand that was co-purified together with the PhnD protein.

The same protein complexed with an unknown ligand, not 2-aminoethyl phosphonate (3P7I). This unknown ligand was co-purified together with PhnD through the protein purification process.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Bind and transport of phosphonates, TRANSPORT PROTEIN

EEQEKALNFGIISTESQQNLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRANGQV
FAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVPGYYVFAKNNISASDFKRTVNAGHETN
ALAVANKQVDVATNNTENLDKLKTSAPEKLKELKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLER
LGWAPFRASSDLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVSKAVQ