Crystal Structure Analysis of H185F Mutant of Human CLIC1

Role of individual histidines in the pH-dependent global stability of human chloride intracellular channel 1.

Chloride intracellular channel protein 1

Chloride channel ABP, Nuclear chloride ion channel 27, NCC27, Regulatory nuclear chloride ion channel protein, hRNCC

MAEEQPQVELFVKAGSDGAKIGNCPFSQRLFMVLWLKGVTFNVTTVDTKRRTETVQKLCPGGQLPFLLYGTEVHTDTNKI
EEFLEAVLCPPRYPKLAALNPESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALKVLDNYLTSPLPEEVDETSAEDE
GVSQRKFLDGNELTLADCNLLPKLFIVQVVCKKYRGFTIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKAL
K

0.1 M Bis-Tris, 30% (v/v) PEG 550 monomethyl ether, 0.05 M calcium chloride, 2.5 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystal Structure Analysis of H207F Mutant of Human CLIC1

Solvent flattening and Histogram matching

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS; U VALUES: WITH TLS ADDED

CLIC, pH sensor, Thioredoxin, GST superfamily, Ion channel, glutathione, cytosol, membrane, TRANSPORT PROTEIN

MAEEQPQVELFVKAGSDGAKIGNCPFSQRLFMVLWLKGVTFNVTTVDTKRRTETVQKLCPGGQLPFLLYGTEVHTDTNKI
EEFLEAVLCPPRYPKLAALNPESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALKVLDNYLTSPLPEEVDETSAEDE
GVSQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRGFTIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKAL
K