RedJ-Thioesterase from the Prodiginine biosynthetic pathway in Streptomyces coelicolor

Structure and Function of the RedJ Protein, a Thioesterase from the Prodiginine Biosynthetic Pathway in Streptomyces coelicolor.

(MSE)HHHHHHSSGVDLGTENLYFQSNALLSQRSAWFPRPVAAPAAEPPDPAAAPLRLVCFPYAGGTVSAFRGWQERLGD
EVAVVPVQLPGRGLRLRERPYDT(MSE)EPLAEAVADALEEHRLTHDYALFGHS(MSE)GALLAYEVACVLRRRGAPRPR
HLFVSGSRAPHLYGDRADHTLSDTALREVIRDLGGLDDADTLGAAYFDRRLPVLRADLRACERYDWHPRPPLDCPTTAFS
AAADPIATPE(MSE)VEAWRPYTTGSFLRRHLPGNHFFLNGGPSRDRLLAHLGTEL

MHHHHHHSSGVDLGTENLYFQSNALLSQRSAWFPRPVAAPAAEPPDPAAAPLRLVCFPYAGGTVSAFRGWQERLGDEVAV
VPVQLPGRGLRLRERPYDTMEPLAEAVADALEEHRLTHDYALFGHSMGALLAYEVACVLRRRGAPRPRHLFVSGSRAPHL
YGDRADHTLSDTALREVIRDLGGLDDADTLGAAYFDRRLPVLRADLRACERYDWHPRPPLDCPTTAFSAAADPIATPEMV
EAWRPYTTGSFLRRHLPGNHFFLNGGPSRDRLLAHLGTEL

12% PEG2000, 0.7 M sodium chloride, 2 mM DTT, 0.1 M HEPES, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Structure of RedJ with PEG bound in the active site.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

LLSQRSAWFPRPVAAPAAEPPDPAAAPLRLVCFPYAGGTVSAFRGWQERLGDEVAVVPVQLPGRGLRLRERPYDTMEPLA
EAVADALEEHRLTHDYALFGHSMGALLAYEVACVLRRRGAPRPRHLFVSGSRAPHLYGDRADHTLSDTALREVIRDLGGL
DDADTLGAAYFDRRLPVLRADLRACERYDWHPRPPLDCPTTAFSAAADPIATPEMVEAWRPYTTGSFLRRHLPGNHFFLN
GGPSRDRLLAHLGTEL