Crystal Structure of Cystathionine gamma-synthase MetB (Cgs) from Mycobacterium ulcerans Agy99 bound to HEPES

Seattle Structural Genomics Center for Infectious Disease (SSGCID)

4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

(2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid

Structure of the cystathionine [gamma]-synthase MetB from Mycobacterium ulcerans

Increasing the structural coverage of tuberculosis drug targets.

GPGSMKDDHKAQHRFAGLATRAIHSGYRPDPATGAVNAPIYASSTFAQDGVGGLRGGYEYARTGNPTRTALEAALAAVED
AAFGRAFSSGMAAADCALRAMLRPGDHVVIPDDAYGGTFRLIDKVFTGWNVEYTPVALADLDAVRAAIRPTTRLIWVETP
TNPLLSIADIAGIAQLGADSSAKVLVDNTFASPALQQPLSLGADVVLHSTT(LLP)YIGGHSDVVGGALVTNDEELDQSF
AFLQNGAGAVPGPFDAYLTMRGLKTLVLRMQRHSENAAAVAEFLAEHPAISTVLYPGLPSHPGHAVAARQMRGFGGMVSV
RMRAGRTAAEQLCAKTNIFILAESLGSVESLIEHPSAMTHASTAGSQLEVPDDLVRLSVGIEDVADLLDDLKQALG

GPGSMKDDHKAQHRFAGLATRAIHSGYRPDPATGAVNAPIYASSTFAQDGVGGLRGGYEYARTGNPTRTALEAALAAVED
AAFGRAFSSGMAAADCALRAMLRPGDHVVIPDDAYGGTFRLIDKVFTGWNVEYTPVALADLDAVRAAIRPTTRLIWVETP
TNPLLSIADIAGIAQLGADSSAKVLVDNTFASPALQQPLSLGADVVLHSTTKYIGGHSDVVGGALVTNDEELDQSFAFLQ
NGAGAVPGPFDAYLTMRGLKTLVLRMQRHSENAAAVAEFLAEHPAISTVLYPGLPSHPGHAVAARQMRGFGGMVSVRMRA
GRTAAEQLCAKTNIFILAESLGSVESLIEHPSAMTHASTAGSQLEVPDDLVRLSVGIEDVADLLDDLKQALG

25.5% PEG4000, 15% glycerol, 170 mM ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 289K

Seattle Structural Genomics Center for Infectious Disease

Crystal Structure of Cystathionine gamma-synthase MetB (Cgs) from Mycobacterium ulcerans Agy99

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Structural Genomics, Seattle Structural Genomics Center for Infectious Disease, SSGCID, CGS_like, AAT_I superfamily, LLP, PLP, LYASE

MKDDHKAQHRFAGLATRAIHSGYRPDPATGAVNAPIYASSTFAQDGVGGLRGGYEYARTGNPTRTALEAALAAVEDAAFG
RAFSSGMAAADCALRAMLRPGDHVVIPDDAYGGTFRLIDKVFTGWNVEYTPVALADLDAVRAAIRPTTRLIWVETPTNPL
LSIADIAGIAQLGADSSAKVLVDNTFASPALQQPLSLGADVVLHSTTKYIGGHSDVVGGALVTNDEELDQSFAFLQNGAG
AVPGPFDAYLTMRGLKTLVLRMQRHSENAAAVAEFLAEHPAISTVLYPGLPSHPGHAVAARQMRGFGGMVSVRMRAGRTA
AEQLCAKTNIFILAESLGSVESLIEHPSAMTHASTAGSQLEVPDDLVRLSVGIEDVADLLDDLKQALG