Crystal structure of the editing domain of threonyl-tRNA synthetase from Pyrococcus abyssi in complex with seryl-3'-aminoadenosine

Mechanistic insights into cognate substrate discrimination during proofreading in translation

MRVLLIHSDYIEYEVKDKALKNPEPISEDMKRGRMEEVLVAFISVEKVDEKNPEEVSLKAIEEISKVAEQVKAENVFVYP
FAHLSSELAKPSVAMDILNRVYQGLKERGFNVGKAPFGYYKAFKISCKGHPLAELSRTIVPEEARVE

PEG 3350, Bis-Tris, NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

SEQUENCE OF THE PROTEIN USED IN THIS STUDY WAS THE FRAGMENT OF UNP RESIDUES 1-183 OF DATABASE Q9UZ14 (SYT_PYRAB). BUT THE C-TERMINAL PROBABLY HAVE BEEN CLEAVED BEFORE CRYSTALLIZING.

alpha/beta fold, deacylase, editing, aminoacyl-tRNA synthetase, translation, LIGASE