Crystal structure of the nucleoside-diphosphate sugar epimerase from Corynebacterium glutamicum. Northeast Structural Genomics Consortium Target CgR91

Northeast Structural Genomics Consortium (NESG)

Nucleoside-diphosphate sugar epimerase (SulA family)

(MSE)SLTTSHFIPFPRE(MSE)VWDWHTRKGAVARLTPPFIPLNPITQAERLADGTTIFSLPAGLKWVARHDLSGFLNG
SRFTDVCLTAPVKALANWRHVHNFVDQDGGTLITDSVSTRLPASTLTG(MSE)FAYRQTQLIEDLKFLSRTSTLFDGSPL
TVAITGSRGLVGRALTAQLQTGGHEVIQLVRKEPKPGKRFWDPLNPASDLLDGADVLVHLAGEPIFGRFNDSHKEAIRES
RVLPTKFLAELVAESTQCTT(MSE)ISASAVGFYGHDRGDEILTEESESGDDFLAEVCRDWEHATAPASDAGKRVAFIRT
GVALSGRGG(MSE)LPLLKTLFSTGLGGKFGDGTSWFSWIAIDDLTDIYYRAIVDAQISGPINAVAPNPVSNAD(MSE)T
KILATS(MSE)HRPAFIQIPSLGPKILLGSQGAEELALASQRTAPAALENLSHTFRYTDIGAAIAHELGYEQLADFAQQQ
EIEAERKQERAELKAAKKIAKKAPVLEESPTNLEDPEEVEQSILSSILNFRRKRNDLEHHHHHH

MSLTTSHFIPFPREMVWDWHTRKGAVARLTPPFIPLNPITQAERLADGTTIFSLPAGLKWVARHDLSGFLNGSRFTDVCL
TAPVKALANWRHVHNFVDQDGGTLITDSVSTRLPASTLTGMFAYRQTQLIEDLKFLSRTSTLFDGSPLTVAITGSRGLVG
RALTAQLQTGGHEVIQLVRKEPKPGKRFWDPLNPASDLLDGADVLVHLAGEPIFGRFNDSHKEAIRESRVLPTKFLAELV
AESTQCTTMISASAVGFYGHDRGDEILTEESESGDDFLAEVCRDWEHATAPASDAGKRVAFIRTGVALSGRGGMLPLLKT
LFSTGLGGKFGDGTSWFSWIAIDDLTDIYYRAIVDAQISGPINAVAPNPVSNADMTKILATSMHRPAFIQIPSLGPKILL
GSQGAEELALASQRTAPAALENLSHTFRYTDIGAAIAHELGYEQLADFAQQQEIEAERKQERAELKAAKKIAKKAPVLEE
SPTNLEDPEEVEQSILSSILNFRRKRNDLEHHHHHH

2% PEG 20000, 0.1M magnesium acetate, 0.1M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K

Northeast Structural Genomics Consortium

DUF1731_C, Northeast Structural Genomics Consortium, NESG, CgR91, PSI-Biology, Protein Structure Initiative, ISOMERASE

MSLTTSHFIPFPREMVWDWHTRKGAVARLTPPFIPLNPITQAERLADGTTIFSLPAGLKWVARHDLSGFLNGSRFTDVCL
TAPVKALANWRHVHNFVDQDGGTLITDSVSTRLPASTLTGMFAYRQTQLIEDLKFLSRTSTLFDGSPLTVAITGSRGLVG
RALTAQLQTGGHEVIQLVRKEPKPGKRFWDPLNPASDLLDGADVLVHLAGEPIFGRFNDSHKEAIRESRVLPTKFLAELV
AESTQCTTMISASAVGFYGHDRGDEILTEESESGDDFLAEVCRDWEHATAPASDAGKRVAFIRTGVALSGRGGMLPLLKT
LFSTGLGGKFGDGTSWFSWIAIDDLTDIYYRAIVDAQISGPINAVAPNPVSNADMTKILATSMHRPAFIQIPSLGPKILL
GSQGAEELALASQRTAPAALENLSHTFRYTDIGAAIAHELGYEQLADFAQQQEIEAERKQERAELKAAKKIAKKAPVLEE
SPTNLEDPEEVEQSILSSILNFRRKRND