Crystal structure of the HIV-2 neutralizing Fab fragment 7C8

Crystal Structure of the HIV-2 Neutralizing Fab Fragment 7C8 with High Specificity to the V3 Region of gp125.

Mouse anti V3 antibody 7C8 Fab, heavy chain

Mouse anti V3 antibody 7C8 Fab, light chain

AVQLEESGPELVKPGALVKISCKASGYTFTTYDINWVKQRPGQGLEWIGWIYPGDGSNKYNEKFKDKATLTVDKSSSTAY
MHLSSLTSENSAVYFCARGYGKNVYAMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVT
WNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRD

DVVMTQTPLSLPVSLGDQASISCRSSQTIVHSNGYTYLDWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKI
SRVEAEDLGVYYCFQGSHVPFTFGSGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSER
QNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE

50 mM ammonium sulphate, 25% (w/v) PEG 8000 and 2.5% (v/v) PEG 400, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Antigen binding, Antibody, Fab, Immunological, HIV-2, V3, gp125, IMMUNE SYSTEM