I-MsoI re-designed for altered DNA cleavage specificity (-8G)

Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs.

DNA (5'-D(*GP*CP*AP*GP*GP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*CP*TP*CP*CP*G)-3')

DNA (5'-D(*CP*GP*GP*AP*GP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*CP*CP*TP*GP*C)-3')

TLQPTEAAYIAGFLDGDGSIYAKLEPRPDYKDIKYQVRLAISFIQRKDKFPYLQDIYDQLGKRGNLRKDRGDGIADYTIY
GSTHLSIILPDLVPYLRIKKKQANRILHIINLYPQAQKNPSKFLDLVKIVDDVQNLNKRADELKSTNYDRLLEEFLKAGK
I

(DG)(DC)(DA)(DG)(DG)(DA)(DC)(DG)(DT)(DC)(DG)(DT)(DG)(DA)(DG)(DA)(DC)(DA)(DG)(DC)
(DT)(DC)(DC)(DG)

(DC)(DG)(DG)(DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DC)(DA)(DC)(DG)(DA)(DC)(DG)(DT)(DC)
(DC)(DT)(DG)(DC)

24% PEG 4000, 100mM Tris, 20mM NaCl, 5mM CaCl2 , pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K

protein-DNA complex, homing nuclease, Rosetta design, DE NOVO PROTEIN-DNA complex