The crystal structure of ASAP3 in complex with Arf6 in transition state soaked with Calcium

The structure of an Arf-ArfGAP complex reveals a Ca2+ regulatory mechanism

SAGITALLY - HORIZONTALLY FOCUSED SI(111) MONOCHROMATOR

Missing the N-terminus amphipathic helix for Arf6
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3, ADP-ribosylation factor 6
GAP and Ankyrin domain, residues 416-697, residues 11-175

ASAP3, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1, Arf6, UPLC1

GHDGEPHDLTKLLIAEVKSRPGNSQCCDCGAADPTWLSTNLGVLTCIQCSGVHRELGVRFSRMQSLTLDLLGPSELLLAL
NMGNTSFNEVMEAQLPSHGGPKPSAESDMGTRRDYIMAKYVEHRFARRCTPEPQRLWTAICNRDLLSVLEAFANGQDFGQ
PLPGPDAQAPEELVLHLAVKVANQASLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLKLLLKGRALVGTVNEAG
ETALDIARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTENLSSDSSLSSPSALNSLSSPSALNSTASNSPGIEGLSN
KEMRILMLGLDAAGKTTILYKLKLGQSVTTIPTVGFNVETVTYKNVKFNVWDVGGQDKIRPLWRHYYTGTQGLIFVVDCA
DRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHEIQEKLGLTRIRDRNWYVQPSCATSGDGLYEGLTWLTS
NYKSKLAAALEHHHHHH

13% PEG 8000, 150mM Magnesium Acetate, 100mM MOPS(pH 7.5); Crystals were then soaked in 30mM CaCl2 , VAPOR DIFFUSION, SITTING DROP, temperature 293K

same protein complex in transition state

NLSSDSSLSSPSALNSLSSPSALNSTASNSPGIEGLS IS A FLEXIBLE LINKER REGION. THIS ENTRY USES NON-SEQUENTIAL NUMBERING.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Arf6, ASAP3, UPLC1, GDP, Calcium, ArfGAP, Arf, Linkers, Alternative splicing, ANK repeat, Coiled coil, Cytoplasm, Metal-binding, Phosphoprotein, Polymorphism, Zinc, Zinc-finger, Cell membrane, Endosome, ER-Golgi transport, Golgi apparatus, GTP-binding, Lipoprotein, Myristate, Nucleotide-binding, Protein transport, Transport

GHDGEPHDLTKLLIAEVKSRPGNSQCCDCGAADPTWLSTNLGVLTCIQCSGVHRELGVRFSRMQSLTLDLLGPSELLLAL
NMGNTSFNEVMEAQLPSHGGPKPSAESDMGTRRDYIMAKYVEHRFARRCTPEPQRLWTAICNRDLLSVLEAFANGQDFGQ
PLPGPDAQAPEELVLHLAVKVANQASLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLKLLLKGRALVGTVNEAG
ETALDIARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTE

NKEMRILMLGLDAAGKTTILYKLKLGQSVTTIPTVGFNVETVTYKNVKFNVWDVGGQDKIRPLWRHYYTGTQGLIFVVDC
ADRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHEIQEKLGLTRIRDRNWYVQPSCATSGDGLYEGLTWLT
SNYKS