Crystal structure of DesT in complex with palmitoyl-CoA

Structural basis for the transcriptional regulation of membrane lipid homeostasis.

MASPRAEQKQQTRHALMSAARHLMESGRGFGSLSLREVTRAAGIVPAGFYRHFSDMDQLGLALVAEVDETFRATLRAVRR
NEFELGGLIDASVRIFLDAVGANRSQFLFLAREQYGGSLPIRQAIASLRQRITDDLAADLALLNKMPHLDGAALDVFADL
VVKTVFATLPELIDPPAADLPPHLMPAAKITHQLRFIMIGGKHWHGLPGSSVDKLAAALE

ML- 0.1M HEPES, pH 7.5, 0.1 M magnesium acetate, 15% PEG 4000. Drop- 20 mg/ml protein, 0.9 mM 16:0-CoA. 2+2 ul drop., VAPOR DIFFUSION, SITTING DROP, temperature 291K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Transcriptional Repressor, DesT, TetR family, DNA-binding, Transcription, Transcription regulation

MSSPRAEQKQQTRHALMSAARHLMESGRGFGSLSLREVTRAAGIVPAGFYRHFSDMDQLGLALVAEVDETFRATLRAVRR
NEFELGGLIDASVRIFLDAVGANRSQFLFLAREQYGGSLPIRQAIASLRQRITDDLAADLALLNKMPHLDGAALDVFADL
VVKTVFATLPELIDPPAADLPPHLMPAAKITHQLRFIMIGGKHWHGLPG