Crystal structure of Reston ebolavirus VP35 RNA binding domain

Ebolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppression.

C-terminal RNA binding domain (UNP residues 160-329)

MAHHHHHHVDDDDKPGPALYEENALKGKIDDPNSYVPDAVQEAYKNLDSTSTLTEENFGKPYISAKDLKEIMYDHLPGFG
TAFHQLVQVICKIGKDNNLLDTIHAEFQASLADGDSPQCALIQITKRVPIFQDVPPPIIHIRSRGDIPRACQKSLRPAPP
SPKIDRGWVCLFKMQDGKTLGLKI

90mM tripostassium citrate, 100mM sodium acetate, 5% (wt/vol) PEG 6000, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K

ebolavirus, RNA-binding protein, protein-RNA complex, interferon, Reston, Host cytoplasm, Interferon antiviral system evasion, RNA replication, RNA-binding, Transcription, Virion, VIRAL PROTEIN, RNA BINDING PROTEIN

PGPALYEENALKGKIDDPNSYVPDAVQEAYKNLDSTSTLTEENFGKPYISAKDLKEIMYDHLPGFGTAFHQLVQVICKIG
KDNNLLDTIHAEFQASLADGDSPQCALIQITKRVPIFQDVPPPIIHIRSRGDIPRACQKSLRPAPPSPKIDRGWVCLFKM
QDGKTLGLKI