Crystal structure of SnoaB, a cofactor-independent oxygenase from Streptomyces nogalater, determined to 1.9 resolution

Crystal structure of the cofactor-independent monooxygenase SnoaB from Streptomyces nogalater: implications for the reaction mechanism

Expression, purification and crystallization of the cofactor-independent monooxygenase SnoaB from the nogalamycin biosynthetic pathway.

MAPTRVNDGVDADEVTFVNRFTVHGAPAEFESVFARTAAFFARQPGFVRHTLLRERDKDNSYVNIAVWTDHDAFRRALAQ
PGFLPHATALRALSTSEHGLFTARQTLPEGGDTTGSGHRHHHHHH

(NH4)2SO4, NaCl, Na-cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystal structure of SnoaB determined to resolution of 1.7 A

Crystal structure of mutant N63A of SnoaB

AUTHORS STATE THAT G25A IS IN FACT THE NATIVE SEQUENCE. THEY CONFIRMED THEIR NATIVE SEQUENCE BY DNA-SEQUENCING THREE TIMES. THIS HAS BEEN NOTED IN THE SECONDARY REFERENCE, KOSKINIEMI ET AL. 2009

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES: RESIDUAL ONLY.

RESIDUAL DENSITY FOR A LIGAND IN THE ACTIVE SITE WAS NOT MODELLED, BECAUSE THE CHEMICAL NATURE OF THIS COMPOUND IS UNKNOWN.

polyketide, anthracycline, oxygenase, cofactor-independent, OXIDOREDUCTASE

MPTRVNDGVDADEVTFVNRFTVHGGPAEFESVFARTAAFFARQPGFVRHTLLRERDKDNSYVNIAVWTDHDAFRRALAQP
GFLPHATALRALSTSEHGLFTARQTLPEGGDTTGSGHR