KINESIN (DIMERIC) FROM RATTUS NORVEGICUS

The crystal structure of dimeric kinesin and implications for microtubule-dependent motility.

Crystallization and Preliminary X-Ray Analysis of the Single-Headed and Double-Headed Motor Protein Kinesin

X-Ray Structure of Motor and Neck Domains from Rat Brain Kinesin

ADPAECSIKVMCRFRPLNEAEILRGDKFIPKFKGEETVVIGQGKPYVFDRVLPPNTTQEQVYNACAKQIVKDVLEGYNGT
IFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIAHDIFDHIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLAVHEDKN
RVPYVKGCTERFVSSPEEVMDVIDEGKANRHVAVTNMNEHSSRSHSIFLINIKQENVETEKKLSGKLYLVDLAGSEKV

NKSLSALGNVISALAEGTKTHVPYRDSKMTRILQDSLGGNCRTTIVICCSPSVFNEAETKSTLMFGQRAKTIKNTVSVNL
ELTAEEWKKKYEKEKEKNKALKSVIQHLEVELNRWRN

HANGING DROP METHOD WAS USED. PROTEIN WAS CRYSTALLIZED FROM 20MM PIPES, PH 7.5, 200MM NACL, 2MM DTT, 2 MM NA-EGTA, 0.8 M AS. THE RESERVOIR CONTAINED 1.6 M AS IN THE SAME BUFFER., vapor diffusion - hanging drop

KINESIN HEAVY CHAIN, ADENOSINE-5'-DIPHOSPHATE