Dehydratase domain from CurF module of Curacin polyketide synthase

Crystal Structures of Dehydratase Domains from the Curacin Polyketide Biosynthetic Pathway.

SNAHPLLGEKINLAGIEDQHRFQSYIGAESPGYLNHHQVFGKVLFPSTGYLEIAASAGKSLFTSQEQVVVSDVDILQSLV
IPETEIKTVQTVVSFAENNSYKFEIFSPSEGENQQTPQWVLHAQGKIYTEPTRNSQAKIDLEKYQAECSQAIEIEEHYRE
YRSKGIDYGSSFQGIKQLWKGQGKALGEMAFPEELTAQLADYQLHPALLDAAFQIVSYAIPHTETDKIYLPVGVEKFKLY
RQTISQVWAIAEIRQTNLTANIFLVDNQGTVLVELEGLRVKVTEP

14% (w/v) PEG 4000, 200 mM CaCl2, 10% (v/v) glycerol, 2 mM DTT, 100 mM Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Dehydratase domain from CurH module of Curacin polyketide synthase

Dehydratase domain from CurJ module of Curacin polyketide synthase

Dehydratase domain from CurK module of Curacin polyketide synthase

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

polyketide synthase, double hotdog fold, dehydratase, LYASE

HPLLGEKINLAGIEDQHRFQSYIGAESPGYLNHHQVFGKVLFPSTGYLEIAASAGKSLFTSQEQVVVSDVDILQSLVIPE
TEIKTVQTVVSFAENNSYKFEIFSPSEGENQQTPQWVLHAQGKIYTEPTRNSQAKIDLEKYQAECSQAIEIEEHYREYRS
KGIDYGSSFQGIKQLWKGQGKALGEMAFPEELTAQLADYQLHPALLDAAFQIVSYAIPHTETDKIYLPVGVEKFKLYRQT
ISQVWAIAEIRQTNLTANIFLVDNQGTVLVELEGLRVKVTEP