Human Eg5 in complex with S-trityl-L-cysteine

Allosteric drug discrimination is coupled to mechanochemical changes in the kinesin-5 motor core.

motor domain of human kinesin Eg5 (residues 1-369)

Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Thyroid receptor-interacting protein 5, TRIP-5, Kinesin-like protein 1

MASQPNSSAKKKEEKGKNIQVVVRCRPFNLAERKASAHSIVECDPVRKEVSVRTGGLADKSSRKTYTFDMVFGASTKQID
VYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGERSPNEEYTWEEDPLAGIIPRTLHQIFEKLTDNGTEFSVKVSL
LEIYNEELFDLLNPSSDVSERLQMFDDPRNKRGVIIKGLEEITVHNKDEVYQILEKGAAKRTTAATLMNAYSSRSHSVFS
VTIHMKETTIDGEELVKIGKLNLVDLAGSENIGRSGAVDKRAREAGNINQSLLTLGRVITALVERTPHVPYRESKLTRIL
QDSLGGRTRTSIIATISPASLNLEETLSTLEYAHRAKNILNKPEVNQKL

250 mM NH4SO4, 100 mM MES pH 6.0, 25% PEG 3350, 5-15% glycerol, 10 mM trimethylamine, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Human Kinesin Eg5 in complex with Mg2+ ADP

Human Kinesin Eg5 in complex with Mg2+ AMPPNP

Human Kinesin Eg5 in complex with monastrol

cell cycle, kinesin-inhibitor complex, motor domain, L5 loop, ATP-binding, Cell division, Coiled coil, Microtubule, Mitosis, Motor protein, Nucleotide-binding, Phosphoprotein