2.14 Angstrom Crystal Structure of Putative Oxidoreductase (ycdW) from Salmonella typhimurium in Complex with NADP

Center for Structural Genomics of Infectious Diseases (CSGID)

4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

SNA(MSE)EIIFYHPTFNAAWWVNALEKALPHARVREWKVGDNNPADYALVWQPPVE(MSE)LAGRRLKAVFVLGAGVDA
ILSKLNAHPE(MSE)LDASIPLFRLEDTG(MSE)GLQ(MSE)QEYAVSQVLHWFRRFDDYQALKNQALWKPLPEYTREEF
SVGI(MSE)GAGVLGAKVAESLQAWGFPLRCWSRSRKSWPGVESYVGREELRAFLNQTRVLINLLPNTAQTVGIINSELL
DQLPDGAYVLNLARGVHVQEADLLAALDSGKLKGA(MSE)LDVFSQEPLPQESPLWRHPRVA(MSE)TPHIAAVTRPAEA
IDYISRTITQLEKGEPVTGQVDRARGY

SNAMEIIFYHPTFNAAWWVNALEKALPHARVREWKVGDNNPADYALVWQPPVEMLAGRRLKAVFVLGAGVDAILSKLNAH
PEMLDASIPLFRLEDTGMGLQMQEYAVSQVLHWFRRFDDYQALKNQALWKPLPEYTREEFSVGIMGAGVLGAKVAESLQA
WGFPLRCWSRSRKSWPGVESYVGREELRAFLNQTRVLINLLPNTAQTVGIINSELLDQLPDGAYVLNLARGVHVQEADLL
AALDSGKLKGAMLDVFSQEPLPQESPLWRHPRVAMTPHIAAVTRPAEAIDYISRTITQLEKGEPVTGQVDRARGY

Salmonella enterica subsp. enterica serovar Typhimurium

Protein solution: 0.3M Sodium chloride, 10mM HEPES (pH 7.5); Screen solution: 0.3M NDSB-211, 30% PEG 3350, 0.2M Sodium acetate, 0.1M Bis-Tris pH 6.5, 10mm NADP, VAPOR DIFFUSION, HANGING DROP, temperature 295K; Cryo: oil, paratone

Center for Structural Genomics of Infectious Diseases

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Glyoxylate/hydroxypyruvate reductase A (E.C.1.1.1.79, 1.1.1.81)

NADP, NAD, Structural Genomics, Center for Structural Genomics of Infectious Diseases, CSGID, OXIDOREDUCTASE

MEIIFYHPTFNAAWWVNALEKALPHARVREWKVGDNNPADYALVWQPPVEMLAGRRLKAVFVLGAGVDAILSKLNAHPEM
LDASIPLFRLEDTGMGLQMQEYAVSQVLHWFRRFDDYQALKNQALWKPLPEYTREEFSVGIMGAGVLGAKVAESLQAWGF
PLRCWSRSRKSWPGVESYVGREELRAFLNQTRVLINLLPNTAQTVGIINSELLDQLPDGAYVLNLARGVHVQEADLLAAL
DSGKLKGAMLDVFSQEPLPQESPLWRHPRVAMTPHIAAVTRPAEAIDYISRTITQLEKGEPVTGQVDRARGY