Human carbonic anhydrase II with a sulfonamide inhibitor

(4-SULFAMOYL-PHENYL)-THIOCARBAMIC ACID O-(2-THIOPHEN-3-YL-ETHYL) ESTER

Atomic resolution studies of carbonic anhydrase II.

Carbonic anhydrase II, CA-II, Carbonate dehydratase II, Carbonic anhydrase C, CAC

MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLK
GGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVV
DVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELM
VDNWRPAQPLKNRQIKASFK

3M ammonium sulfate, 50 mM tris-Cl 2mM pHMB , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

ELECTRON DENSITY WAS OBSERVED FOR ONLY THE HG AND C1 ATOMS OF A SECOND CONFORMER OF THE HGB LIGAND. PRESUMABLY THE LIGAND IS SUFFICIENTLY DISORDERED BEYOND C1 TO RENDER IT UNOBSERVED

carbonic anhydrase, atomic resolution, sulfonamide inhibitor, Disease mutation, Lyase, Metal-binding, LYASE-LYASE INHIBITOR complex