Crystal structure of macrophage migration inhibitory factor (mif) with hydroxyquinoline inhibitor 707 at 1.58a resolution

Discovery of covalent inhibitors for MIF tautomerase via cocrystal structures with phantom hits from virtual screening.

MIF, Phenylpyruvate tautomerase, L-dopachrome tautomerase, L-dopachrome isomerase, Glycosylation-inhibiting factor, GIF

PMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLC
GLLAERLRISPDRVYINYYDMNAANVGWNNSTFALEHHHHHH

1.5 M AMMONIUM SULFATE, 2 mM EDTA, 0.1 M HEPES, PH 6.5, VAPOR DIFFUSION/HANGING DROP, TEMPERATURE 298.0K , VAPOR DIFFUSION, HANGING DROP

protein-ligand complex, Cytokine, Cytoplasm, Immune response, Inflammatory response, Innate immunity, Isomerase, Phosphoprotein, Secreted

PMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLC
GLLAERLRISPDRVYINYYDMNAANVGWNNSTFA