Crystal structure of Fis bound to 27 bp DNA F29 containing 5 G/Cs at center

The shape of the DNA minor groove directs binding by the DNA-bending protein Fis.

MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNGQDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALM
MGINRGTLRKKLKKYGMN

(DA)(DA)(DA)(DT)(DT)(DT)(DG)(DT)(DT)(DT)(DG)(DG)(DG)(DC)(DG)(DC)(DT)(DG)(DA)(DG)
(DC)(DA)(DA)(DA)(DT)(DT)(DT)

(DA)(DA)(DA)(DT)(DT)(DT)(DG)(DC)(DT)(DC)(DA)(DG)(DC)(DG)(DC)(DC)(DC)(DA)(DA)(DA)
(DC)(DA)(DA)(DA)(DT)(DT)(DT)

0.25 M Sodium citrate, 0.1 M TRIS-HCl pH 8.5, 40% PEG 400, vapor diffusion, hanging drop, temperature 277K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

biological unit is the same as asymmetric unit

Protein-DNA complex, HTH domain, minor groove compression, DNA bending, indirect recognition, activator, DNA-binding, Transcription, Transcription regulation, DNA BINDING PROTEIN-DNA COMPLEX