The structure of the mammalian Sec61 channel opened by a signal sequence

Structure of the Sec61 channel opened by a signal sequence.

DETAILS--Real-space fitting using Chimera and Coot followed by reciprocal-space refinement using REFMAC

50 mM HEPES, 200 mM potassium acetate, 15 mM magnesium acetate, 1 mM DTT, 0.25% Digitonin

Sec61 protein conducting channel opened by the pre-prolactin signal sequence

Quantifoil (R2/2) holey carbon grid covered in a 70 Angstrom-thick layer of amorphous carbon

3 uL sample was added to the grid, incubated for 30 seconds at 4 C, blotted for 9 seconds, and then plunged into liquid ethane (FEI VITROBOT).
3 uL sample was added to the grid, incubated for 30 seconds at 4 C, blotted for 9 seconds

Protein transport protein Sec61 subunit alpha isoform 1

Protein transport protein Sec61 subunit gamma

Protein transport protein Sec61 subunit beta

MAIKFLEVIKPFCVILPEIQKPERKIQFKEKVLWTAITLFIFLVCCQIPLFGIMSSDSADPFYWMRVILASNRGTLMELG
ISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFGMIITIGQSIVYVMTGMYGDPSEMGAGICLLITIQLFVAGL
IVLLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTVNTGRGMEFEGAIIALFHLLATRTDKVRALREAFYRQNLP
NLMNLIATIFVFAVVIYFQGFRVDLPIKSARYRGQYNTYPIKLFYTSNIPIILQSALVSNLYVISQMLSARFSGNLLVSL
LGTWSDTSSGGPARAYPVGGLCHYLSPPESFGSVLEDPVHAVVYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMV
MRGHRETSMVHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQSEVGSMGALLF

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reticulocyte translation extract in the presence of canine microsomes

Protein transport protein Sec61 subunit alpha isoform 1, Protein transport protein Sec61 subunit gamma, Sec61 beta, Prolactin

Sec61, translocation, signal sequence, TRANSPORT PROTEIN