Electron cryo-microscopy of a virus-like particle of orange-spotted grouper nervous necrosis virus

Structural analysis and insertion study reveal the ideal sites for surface displaying foreign peptides on a betanodavirus-like particle

(Single particle details: The particles were selected manually.) (Single particle--Applied symmetry: I)

Orange-spotted grouper nervous necrosis virus

Objective lens astigmatism was corrected at 200000 times magnification

300 mesh Quantifoil copper grid with thin carbon support, glow discharged in air atmosphere

virus-like particle of orange-spotted grouper nervous necrosis virus

MVRKGEKKLAKPATTKAANPQPRRRANNRRRSNRTDAPVSKASTVTGFGRGTNDVHLSGMSRISQAVLPAGTGTDGYVVV
DATIVPDLLPRLGHAARIFQRYAVETLEFEIQPMCPANTGGGYVAGFLPDPTDNDHTFDALQATRGAVVAKWWESRTVRP
QYTRTLLWTSSGKEQRLTSPGRLILLCVGNNTDVVNVSVLCRWSVRLSVPSLETPEETTAPIMTQGSLYNDSLSTNDFKS
ILLGSTPLDIAPDGAVFQLDRPLSIDYSLGTGDVDRAVYWHLKKFAGNAGTPAGWFRWGIWDNFNKTFTDGVAYYSDEQP
RQILLPVGTVCTRVDSEN

THE SEQUENCE OF THIS PROTEIN WAS NOT AVAILABLE AT THE UNIPROT KNOWLEDGEBASE DATABASE (UNIPROTKB) AT THE TIME OF DEPOSITION.

icosahedral asymmetric unit, std point frame

virus-like particle, orange-spotted grouper nervous necrosis virus, betanodavirus, VIRUS