Atomic model of transcribing cytoplasmic polyhedrosis virus

A putative ATPase mediates RNA transcription and capping in a dsRNA virus.

Cytoplasmic polyhedrosis virus with SAM and 4 NTPs

Objective lens astigmatism was corrected at 135,000 times magnification.

Plunged into liquid ethane (FEI VITROBOT MARK II)

MWHYTSINNDTRVALDPKPNQIRTITKPNTVPQLGTDYLYTFNSQRRSHTLRLLGPFQYFNFSETDRGHPLFRLPLKYPS
KAIPADELIDNLHSWMRSVHLLHVRSEDNTLRYNWMLGVYARSTNYTTPVGQLVVNAPAILNYSNPQDAFNSVFVALGID
YIDIPITNSNIFDDSSTPYNVRIWHAPTMTEVNHILALMRKSTLVSTHSSWHWNVLHTFHYRSESDMIDHFAAKILEDWR
QKEKLDKGALVEADRVIQRLIPLSSSTYVQRLAAIGALYPNEFTENVLDLSRLSTALLQLSDTYYQHANDQLRRLYRRMY
NDSRTLYMTQRHQELLLAQITADPNILLYPYTYIFTTIPTSMNYISNTGQGRIKHSLTVTGATEHDTVADIVLGQTGEDV
ITISMVEPMSIAVEDMYGYVLDTPTRDIWPADEQIEQKGDAVALYDTKTSRALGMFNNTVRIDDLLSPLLSLVYRTYIKG
DTMTMTQGSLDHLTLCAAVDSDITFVGNRMIAPLPEGYIPKPMHRNNSTMKMLSLYVALKKLENFATNSYLMAPDTSIIL
LGAEREPAVNILRRFNRNVSNVRIIGMGDRAVEPNIRVRVPFPIDKNISADFIICDINSYEDQSFESMFSETISVVTTCA
SAATRALVKINHPSEYMINSVIERLSQLGGVFYHTALLKTASQNPYSYETYIYITPIAAAVRFPFYSNSAMINRYMTAVA
DDEMPIIPSIHTVIKGHSNTYSPGLFCGCVDVQSAPLALSQLKSYCSEATTWRVDSDDNLVNIIARIDPARIALEFRTRS
NTSAYHEYQRYVPNGLGFKVRKTREFRYMHREVTFIHKLMMYALIREQISLTENMTQVVSIGGRNLADISVVPLNMKYVV
IDPATRIETLTQEKKNIEVQSRPFQFDAANMDLENNSIYLFIAVIMNEPNGAATPARMQMDKIRNVATAMLTRTNCVAYI
SFYEAGIITRLDQSTAHKTIRVEEGRLKVANYVPVDTLVEADVTLMLRDIGITHEIIRPSTPELIDACSNYGIRLGSTGG
AVLDVFNHYSPVIKLVRS

MHSTNNNSNKRNNEEKHKQPEIDSSANNGEGTSGTRAQTVGDTATEAGVRNETEAGASTRRQTDGTGLSGTNAKIATASS
ARQADVEKPADVTFTIENVDDVGIMQQKKPPTVVQSRTDVFNEQFANEALHPTTKVIFNGLDVNTEVQPLSDDFKQISDP
KGYLTYSVKYEDQFTKKDKLRASEADDRIVGPTVNLFKYGAAVVNIDLNRDFFDTATGIDLTKGIPLVQDLLVPIGVTAG
AEQSAEYVSGLLMVLFKVMTDNRLVIVGETTTPMSNTLSTVVNNVLRTTYHNNVGVNPALLRDFTQVNWLNRDITNMLQQ
AGTKYGLGLTETRLDYVRLVKTIVGHALNIDHFAASVLNINLRALMEANVTADDRIKALQAHSMISTQFHGPNQGALRPE
LAFDHDHIIRCLMLAAANYPRLEGIIVQINTGYVASANVIRPVSEKRYFPENLEQNQSAARLVSAVKARASEADISSIHL
AIAREVSPMFNVHELKKIAESFEDPSSIVVVLEFILFALFFPTEFNRIKGDIQNVLLLFFSRWYPVEYGIFVQRGATYTI
NAAGEFEFSGRNEKWDQALYLSEHFPALFSDVPLAGANTIIAIMRLFTPQGFLRTDDLAIAANFPRASRNPQTYIPYTNQ
RGTVTNEFASRFRTIVATLANVVNERAVQDDMQKATRSCTKQWLRHLETQFDNIAVAHTDHLSVVYATMSNFMLNFTNNF
SGNHATFKPDQYVITSPEGSYKPIIERQGETVDGLTIIDTSIVWPILCQCTYPLVRQSGKGVDAVSIMEEIVYPDPSTTL
SQSLSVAQVLSKLTLPDAFINMILSGGDSVVMRTYQTEADDDLDEGIRMTTYDQYLSHIRERLHITNVPDPIYITGASTP
DQIAASVQATHVAVVLYQSGVINGPASTYLRENEVLVVMPDYYDVVSRFANANLQMNNNRYHESVLEIADIFDQADFIQT
SDAVRQLRALMPTLSTSQIRHAIERIAQITDVDSTDYGKLTLRFLGTLTRSLKMQNAQIRRIRPDGTVLRYDDQIDIEAF
RWSRYFLDELQLRRLSVGLRLITNPRIARRFNGVRIMYLTDDDPDPDFVPDVPEGYVAVQYAHRLFSSSLANKRNRVTYT
HPPTGMAYPSPTGRPHVHMTINERAGMSKLVADNIIASVIKSNWVVDILDIEYTAEVMTPSEGYTQHVDAESIMTAPKGK
LFHLQFMDGLLRPEPSAFDPPASGEDMRLIYPLQPISVARSMRAIVNHNEVDRPRGAVAPSSYEMDTGTLSRNGDLLYSP
VANGQVGIPKLEVDHISFSNVVSMMTANIRTGDDMAVERVNPDDVRAINIRNA

MLQQPTGGYTTLEQFAFTIRNDGTNATPTQFLQLLSYEATENELVKKTIPTPETHLPSARNVPGNVYIEDAITQALFGIS
AQNVNAHGYFSRLSALALPNTSARLGLDGVIYNSETINIPFYDPAAVANFAATYAKLGNASTPRYRADMIDIYAHVGLEL
AGTDAERAAGVMPVKRAKFDSWEGSLISLSRDVVNWKILAFLIDLCSLEGEALRAFKTRNRDVFRMMLFIMSTAVAANVV
NRKVTKRVDRVLEYIGVNSMRTAGRTATITYDLSRHEFAAKFLQLTFTRWNAASAMIRSMPDMHTPRTSITPAGENALVR
HNRYMTENFKGLSPIALAQKKHEMMLHTHEIHSMDIDGSIKNMVERETVNKMNEIDAMNTAPWTEEFAEVEPTTVYERHQ
IGTDPEQTQLISQDAAVIVHQASSDVDENEYGNSVSELTIDTQSDSVL

icosahedral asymmetric unit, std point frame
viral ATPase, histidine-mediated guanylyl transfer, conformational changes, regulation of transcription, VIRUS