Cryo-EM structure of 9-fold symmetric rATcpn-beta in apo state

Flexible interwoven termini determine the thermal stability of thermosomes.

METHOD--symmetry-restrained MDFF REFINEMENT PROTOCOL--Rigid body and Molecular Dynamics Flexible Fitting

9-fold symmetric rATcpn-beta in apo state

This sample was applied to a 400-mesh GiG grid with holes of 2 um diameter and 2 um spacing.

Blot for 4 seconds before plunging into liquid ethane (FEI Vitrobot Mark IV).

MASTATVATTPEGIPVIILKEGSSRTYGKEAVRANIAAVKAVEEALKSTYGPRGMDKMLVDSLGDITITNDGATILDKMD
LQHPAAKLLVQIAKGQDEETADGTKTAVIFSGELVKKAEDLLYKDVHPTIIISGYKKAEEVALQTIQELAQTVSINDTDL
LRKIAMTSLSSKAVAGAREYIADIVVKAVTQVAELRGDKWYVDLDNIQIVKKAGGSINDTQLVYGIVVDKEVVHPGMPKR
LENAKIALIDASLEVEKPELDAEIRINDPTQMQKFLDEEENLIKEKVDKILATGANVIICQKGIDEVAQSYLAKKGVLAV
RRAKKSDLEKLARATGGRVVSNIDEISEQDLGYASLIEERKVGEDKMVFVEGAKNPKSISILIRGGLERLVDETERALRD
ALGTVADVIKDGRAIAGGGAVEIEIAKKLRKYAPQVGGKEQLAVEAYANALESLVSILIENAGFDPIDLLMKLRSTHENE
NNKWYGIDLYAGQPVDMWQKGVIEPALVKMNAIKAATEAATLVLRIDDVVSAGKKSGGESKTPGGANKPSEED

Cryo-EM structure of 8-fold symmetric rATcpn-alpha in apo state

Cryo-EM structure of 9-fold symmetric rATcpn-alpha in apo state

Cryo-EM structure of 8-fold symmetric rATcpn-alpha in ATP-binding state

Cryo-EM structure of 9-fold symmetric rATcpn-beta in ATP-binding state

Cryo-EM structure of 9-fold symmetric lidless rATcpn-beta in ATP-binding state

Cryo-EM structure of 9-fold symmetric chimeric group II chaperonin rATcpn-AaIaEb in apo state

Cryo-EM structure of 8-fold symmetric chimeric group II chaperonin rATcpn-AaIbEb in apo state

Cryo-EM structure of 9-fold symmetric chimeric group II chaperonin rATcpn-AaIbEb in apo state

Cryo-EM structure of D3 symmetric heteromeric group II chaperonin rATcpn-ab in apo state

Cryo-EM structure of C3 symmetric heteromeric group II chaperonin rATcpn-ab in apo state