Crystal structure of mono-zinc form of succinyl-diaminopimelate desuccinylase from Haemophilus influenzae

Midwest Center for Structural Genomics (MCSG)

Structural basis for catalysis by the mono- and dimetalated forms of the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase.

SDAP desuccinylase, N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

MKEKVVSLAQDLIRRPSISPNDEGCQQIIAERLEKLGFQIEWMPFNDTLNLWAKHGTSEPVIAFAGHTDVVPTGDENQWS
SPPFSAEIIDGMLYGRGAADMKGSLAAMIVAAEEYVKANPNHKGTIALLITSDEEATAKDGTIHVVETLMARDEKITYCM
VGEPSSAKNLGDVVKNGRRGSITGNLYIQGIQGHVAYPHLAENPIHKAALFLQELTTYQWDKGNEFFPPTSLQIANIHAG
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLEKHNLKYRIEWNLSGKPFLTKPGKLLDSITSAIEETIGITPKAE
TGGGTSDGRFIALMGAEVVEFGPLNSTIHKVNECVSVEDLGKCGEIYHKMLVNLLDS

Ammonium sulfate, 0.2 M NaCl,
0.1 M Na Acetate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystal structure of zinc-bound succinyl-diaminopimelate desuccinylase from Haemophilus influenzae

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

DapE, succinyl-diaminopimelate desuccinylase, Zn-binding, metallopeptidase, Structural Genomics, PSI-2, Protein Structure Initiative, Midwest Center for Structural Genomics, MCSG, Amino-acid biosynthesis, Cobalt, Diaminopimelate biosynthesis, Hydrolase, Lysine biosynthesis, Metal-binding