1.99 Angstrom resolution crystal structure of a short chain dehydrogenase from Bacillus anthracis str. 'Ames Ancestor'

Center for Structural Genomics of Infectious Diseases (CSGID)

SNA(MSE)KEKVVIITGGSSG(MSE)GKG(MSE)ATRFAKEGARVVITGRTKEKLEEAKLEIEQFPGQILTVQ(MSE)DV
RNTDDIQK(MSE)IEQIDEKFGRIDILINNAAGNFICPAEDLSVNGWNSVINIVLNGTFYCSQAIGKYWIEKGIKGNIIN
(MSE)VATYAWDAGPGVIHSAAAKAGVLA(MSE)TKTLAVEWGRKYGIRVNAIAPGPIERTGGADKLWISEE(MSE)AKR
TIQSVPLGRLGTPEEIAGLAYYLCSDEAAYINGTC(MSE)T(MSE)DGGQHLHQYPF

SNAMKEKVVIITGGSSGMGKGMATRFAKEGARVVITGRTKEKLEEAKLEIEQFPGQILTVQMDVRNTDDIQKMIEQIDEK
FGRIDILINNAAGNFICPAEDLSVNGWNSVINIVLNGTFYCSQAIGKYWIEKGIKGNIINMVATYAWDAGPGVIHSAAAK
AGVLAMTKTLAVEWGRKYGIRVNAIAPGPIERTGGADKLWISEEMAKRTIQSVPLGRLGTPEEIAGLAYYLCSDEAAYIN
GTCMTMDGGQHLHQYPF

20% MPD, 0.1M Mg Acetate, 50mM MES pH 5.6. For cryoprotection, 5% Glycerol, 5% Sucrose, 5% Ethylene glycol were used, VAPOR DIFFUSION, SITTING DROP, temperature 295K

Center for Structural Genomics of Infectious Diseases

1. The Friedel pairs were used in phasing. 2. HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Short Chain Dehydrogenase, Structural Genomics, Infectious Diseases, Center for Structural Genomics of Infectious Diseases, Oxidoreductase, CSGID

MKEKVVIITGGSSGMGKGMATRFAKEGARVVITGRTKEKLEEAKLEIEQFPGQILTVQMDVRNTDDIQKMIEQIDEKFGR
IDILINNAAGNFICPAEDLSVNGWNSVINIVLNGTFYCSQAIGKYWIEKGIKGNIINMVATYAWDAGPGVIHSAAAKAGV
LAMTKTLAVEWGRKYGIRVNAIAPGPIERTGGADKLWISEEMAKRTIQSVPLGRLGTPEEIAGLAYYLCSDEAAYINGTC
MTMDGGQHLHQYPF