Structure and function of an iterative polyketide synthase thioesterase domain catalyzing Claisen cyclization in aflatoxin biosynthesis.

Aflatoxin biosynthesis polyketide synthase
PksA Thioesterase Domain residues 1845-2109

LKPYCRPSTSVVLQGLPMVARKTLFMLPDGGGSAFSYASLPRLKSDTAVVGLNCPYARDPENMNCTHGAMIESFCNEIRR
RQPRGPYHLGGWSSGGAFAYVVAEALVNQGEEVHSLIIIDAPIPQAMEQLPRAFYEHCNSIGLFATQPGASPDGSTEPPS
YLIPHFTAVVDVMLDYKLAPLHARRMPKVGIVWAADTVMDERDAPKMKGMHFMIQKRTEFGPDGWDTIMPGASFDIVRAD
GANHFTLMQKEHVSIISDLIDRVMA

0.2 M ammonium acetate, 0.1 M sodium citrate, 30% PEG 4K, pH 5.6, VAPOR DIFFUSION, temperature 298K

a/b hydrolase, thioesterase, norsolorinic acid, aflatoxin, PksA, polyketide, Acyltransferase, Multifunctional enzyme, Phosphopantetheine, HYDROLASE