Crystal structure of the S18Y variant of ubiquitin carboxy terminal hydrolase L1 bound to ubiquitin vinylmethylester.

Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation.

Ubiquitin carboxyl-terminal hydrolase isozyme L1

UCH-L1, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5

GPLGSMQLKPMEINPEMLNKVLYRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEV
SPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVD
DKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA

RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, UBIQ_HUMAN

2.4 M ammonium sulfate, 0.1M BICINE, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

PDB ENTRY 2ETL PDB ENTRY 1XD3, CHAIN B

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

the biological unit is a heterodimer consist of chain A and B in the asymmetric unit.

Enzyme-suicide substrate complex, Cytoplasm, Disease mutation, Glycoprotein, Hydrolase, Ligase, Oxidation, Polymorphism, Protease, Thiol protease, Ubl conjugation pathway, Isopeptide bond, Nucleus, Phosphoprotein, Ubl conjugation, hydrolase-hydrolase inhibitor COMPLEX

MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVY
FMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNF
HFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA