Crystal Structure of catalytic fragment of E. coli AlaRS in complex with AlaSA

4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma.

N-terminal Catalytic fragment residues 2-442

SKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENV
GYTARHHTFFEMLGNFSFGDYFKLDAILFAWLLLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNK
GAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGL
ERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHG
NMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDT
YGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGF

30% PEG 400, 0.1 M HEPES, pH 7.8, vapor diffusion, sitting drop, temperature 277K

Same protein in complex with AMPPCP, Ala-AMP and PCP

Same protein with G237A mutation, in complex with AlaSA

Same protein with AlaRS G237A mutation, in complex with GlySA

Same protein with G237A mutation, in complex with SerSA

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Aminoacyl-tRNA synthetase, Ligase, Protein biosynthesis, Nucleotide-binding, amino acid-binding, ATP-binding, Metal-binding, Zinc-finger

SKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENV
GYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNK
GAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGL
ERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHG
NMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDT
YGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGF