Crystal structure of nucleotide-free hexameric ClpX

Structures of asymmetric ClpX hexamers reveal nucleotide-dependent motions in a AAA+ protein-unfolding machine.

ATP-dependent Clp protease ATP-binding subunit clpX

SALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNGDTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFT
MADATTLTEAGYVGEDVENIIQKLLQKCDYDVQKAQRGIVYIDQIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVAA
VPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKAKSDKASEGELLAQVEPEDLIKFGLI
PEFIGRLPVVATLNELSEEALIQILKEPKNALTKQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALL
DTMYDLPSMEDVEKVVIDESVIDGQSKPLLIYGKPEAQQASGE

75 mM Sodium acetate, 1.9 M ammonium sulfate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K

ClpX, AAA+ molecular machine, hexamer, asymmetric, ATP-binding, Chaperone, Metal-binding, Nucleotide-binding, Stress response, Zinc-finger, METAL BINDING PROTEIN, MOTOR PROTEIN
chain B and (backbone or name CB) and (resi 67-97 or resi 115-144 or resi 161-189 or resi 242-260 or resi 285-308)
chain A and (backbone or name CB) and (resi 67-97 or resi 115-144 or resi 161-189 or resi 242-260 or resi 286-308)
chain C and (backbone or name CB) and (resi 67-97 or resi 115-144 or resi 161-189 or resi 242-260 or resi 285-308)
chain D and (backbone or name CB) and (resi 67-97 or resi 115-144 or resi 161-189 or resi 242-260 or resi 285-308)
chain E and (backbone or name CB) and (resi 67-97 or resi 115-144 or resi 176-189 or resi 242-260 or resi 285-308)
chain F and (backbone or name CB) and (resi 67-97 or resi 115-144 or resi 161-189 or resi 242-260 or resi 285-308)
chain B and (backbone or name CB) and (resi 319-412)
chain A and (backbone or name CB) and (resi 319-412)
chain C and (backbone or name CB) and (resi 319-412)
chain D and (backbone or name CB) and (resi 319-412)
chain E and (backbone or name CB) and (resi 319-412)
chain F and (backbone or name CB) and (resi 319-412)

SALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNGDTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFT
MADATTLTEAGYVGEDVENIIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVAA
VPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKAKSDKASEGELLAQVEPEDLIKFGLI
PEFIGRLPVVATLNELSEEALIQILKEPKNALTKQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALL
DTMYDLPSMEDVEKVVIDESVIDGQSKPLLIYGKPEAQQASGE