2.0 Angstrom Structure of the Ile74Val Variant of Vivid (VVD).

Mechanism-based tuning of a LOV domain photoreceptor.

GSHMHTLYAPGGYDIMGYLIQIMNRPNPQVELGPVDTSCALVLCDLKQKDTPIVYASEAFLYMTGYSNAEVLGRNCRFLQ
SPDGMVKPKSTRKYVDSNTINTMRKAIDRNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE

Protein at 3.6 mg/ml dissolved in Buffer containing 5 mM DTT, 100 mM NaCl, 50 mM HEPES pH 8.0 and 10% glycerol is combined with an equal volume of 28% PEG 4k, 100 mM ammonium acetate, 100 mM trisodium citrate pH 5.6., VAPOR DIFFUSION, HANGING DROP, temperature 298K

1.8 Angstrom Crystal Structure of the I74V:I85V Variant of Vivid (VVD)

2.0 Angstrom Structure of the Wild Type Dark-State of VVD

1.8 Angstrom Structure of the Cys71Ser Variant of VVD

2.3 Angstrom Structure of Phosphodiesterase Treated VVD

1.7 Angstrom Structure of the Wild Type Light-State of VVD

1.65 Angstrom Structure of the Cys71Val Variant of VVD

The protein in monomeric, however following photo-excitation the protein dimerizes. The biological dimer structure is currently unknown.

Photoreceptor, Circadian Clock, FAD, LOV, PAS, SIGNALING PROTEIN

HTLYAPGGYDIMGYLIQIMNRPNPQVELGPVDTSCALILCDLKQKDTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDG
MVKPKSTRKYVDSNTINTMRKAIDRNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE