The first X-ray structure of a sulfide:quinone oxidoreductase: Insights into sulfide oxidation mechanism

Structural and functional insights into sulfide:quinone oxidoreductase.

MTKVLVLGGRFGALTAAYTLKRLVGSKADVKVINKSRFSYFRPALPHVAIGVRDVDELKVDLSEALPEKGIQFQEGTVEK
IDAKSSMVYYTKPDGSMAEEEYDYVIVGIGAHLATELVKGWDKYGYSVCEPEFATKLREKLESFQGGNIAIGSGPFYQGH
NPKPKVPENFVPNADSACEGPVFEMSLMLHGYFKKKGMLDKVHVTVFSPGEYLSDLSPNSRKAVASIYNQLGIKLVHNFK
IKEIREHEIVDEKGNTIPADITILLPPYTGNPALKNSTPDLVDDGGFIPTDLNMVSIKYDNVYAVGDANSMTVPKLGYLA
VMTGRIAAQHLANRLGVPTKVDKYYPTIVCVADNPYEGYAVSVKDDTWYGGTVSIADPAAVNHLKKELFTKYYMWTKGDM
ALEKFLASW

2.2 M NH4H2PO4, 100 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

2.2 M NH4H2PO4/K2HPO4, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

ACCORDING TO THE AUTHORS THE UNP DATABASE SEQUENCE IS IN ERROR AT THIS POSITION

Membrane protein, rossman-like fold, Oxidoreductase

MTKVLVLGGRFGALTAAYTLKRLVGSKADVKVINKSRFSYFRPALPHVAIGVRDVDELKVDLSEALPEKGIQFQEGTVEK
IDAKSSMVYYTKPDGSMAEEEYDYVIVGIGAHLATELVKGWDKYGYSVCEPEFATKLREKLESFQGGNIAIGSGPFYQGH
NPKPKVPENFVPNADSACEGPVFEMSLMLHGYFKKKGMLDKVHVTVFSPGEYLSDLSPNSRKAVASIYNQLGIKLVHNFK
IKEIREHEIVDEKGNTIPADITILLPPYTGNPALKNSTPDLVDDGGFIPTDLNMVSIKYDNVYAVGDANSMTVPKLGYLA
VMTGRIAAQHLANRLGVPTKVDKYYPTIMCVADNPYEGYAVSVKDDTWYGGTVSIADPAAVNHLKKELFTKYYMWTKGDM
ALEKFLASW