Crystal Structure of the methyltransferase BC_2162 in complex with S-Adenosyl-L-Homocysteine from Bacillus cereus, Northeast Structural Genomics Consortium Target BcR20

Northeast Structural Genomics Consortium (NESG)

Northeast Structural Genomics Consortium Target BcR20

Substitution of Met residues by Seleno-Met residues and addition of a C-tag (lehhhhhh).

RDLYYNDDYVSFLVNTVWKITKPVHIVDYGCGYGYLGLVL(MSE)PLLPEGSKYTGIDSGETLLAEARELFRLLPYDSEF
LEGDATEIELNDKYDIAICHAFLLH(MSE)TTPET(MSE)LQK(MSE)IHSVKKGGKIICFEPHWISN(MSE)ASYLLDG
EKQSEFIQLGVLQKLFESDTQRNGKDGNIG(MSE)KIPIYLSELGVKNIECRVSDKVNFLDSN(MSE)HHNDKNDLYQSL
KEEGIAGDPGDKQQFVERLIARGLTYDNALAQYEAELRFFKALHLHSSLVYAPN(MSE)KITFGEIECLEHHHHHH

RDLYYNDDYVSFLVNTVWKITKPVHIVDYGCGYGYLGLVLMPLLPEGSKYTGIDSGETLLAEARELFRLLPYDSEFLEGD
ATEIELNDKYDIAICHAFLLHMTTPETMLQKMIHSVKKGGKIICFEPHWISNMASYLLDGEKQSEFIQLGVLQKLFESDT
QRNGKDGNIGMKIPIYLSELGVKNIECRVSDKVNFLDSNMHHNDKNDLYQSLKEEGIAGDPGDKQQFVERLIARGLTYDN
ALAQYEAELRFFKALHLHSSLVYAPNMKITFGEIECLEHHHHHH

Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), and 10mM S-Adenosyl-L-Methionine . Reservoir solution: 100 mM Sodium Citrate and 5.76M Potassium Acetate , VAPOR DIFFUSION, HANGING DROP, temperature 277K

Northeast Structural Genomics Consortium

2GH1 is the crystal structure of the apo enzyme.

alpha-beta protein, Structural Genomics, PSI-2, Protein Structure Initiative, Northeast Structural Genomics Consortium, NESG, Methyltransferase, Transferase

RDLYYNDDYVSFLVNTVWKITKPVHIVDYGCGYGYLGLVLMPLLPEGSKYTGIDSGETLLAEARELFRLLPYDSEFLEGD
ATEIELNDKYDIAICHAFLLHMTTPETMLQKMIHSVKKGGKIICFEPHWISNMASYLLDGEKQSEFIQLGVLQKLFESDT
QRNGKDGNIGMKIPIYLSELGVKNIECRVSDKVNFLDSNMHHNDKNDLYQSLKEEGIAGDPGDKQQFVERLIARGLTYDN
ALAQYEAELRFFKALHLHSSLVYAPNMKITFGEIEC