Crystal Structure of ApcT Transporter Bound to 7F11 Monoclonal Fab Fragment

New York Consortium on Membrane Protein Structure (NYCOMPS)

Structure and mechanism of a na+-independent amino Acid transporter.

7F11 Anti-ApcT Monoclonal Fab Light Chain

7F11 Anti-ApcT Monoclonal Fab Heavy Chain

DIVMTQSPSSLAMSVGQKVTLSCKSSQSLLNTSNQKNYLAWYQQKPGQSPKLLVYFASTRESGVPDRFIGSGSGTDFTLT
ISSVQAEDLSDFFCQQHYSTPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSE
RQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

QVQLLQPGAELVKPGASVKLSCKASGYTFTSYWMYWVKQRPGQGLEWIGEIDPSDSYTNYNQNFKGKATLTVDKSSSTAF
MQLSSLTSQDSAVYFCARSPHYYGTTYNYPMDYWGQGTSVTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPES
VTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEP

MELKNKKLSLWEAVSMAVGVMIGASIFSIFGVGAKIAGRNLPETFILSGIYALLVAYSYTKLGAKIVSNAGPIAFIHKAI
GDNIITGALSILLWMSYVISIALFAKGFAGYFLPLINAPINTFNIAITEIGIVAFFTALNFFGSKAVGRAEFFIVLVKLL
ILGLFIFAGLITIHPSYVIPDLAPSAVSGMIFASAIFFLSYMGFGVITNASEHIENPKKNVPRAIFISILIVMFVYVGVA
ISAIGNLPIDELIKASENALAVAAKPFLGNLGFLLISIGALFSISSAMNATIYGGANVAYSLAKDGELPEFFERKVWFKS
TEGLYITSALGVLFALLFNMEGVASITSAVFMVIYLFVILSHYILIDEVGGRKEIVIFSFIVVLGVFLLLLYYQWITNRF
VFYGIIATFIGVLIFEIIYRKVTKRTFSNNMYVKSLESSGLVPR

0.1M sodium acetate, 0.1M rubidium chloride, 0.1M L-alanine, 40-48% PEG 400, pH 5.0, Microbatch, temperature 293K

New York Consortium on Membrane Protein Structure

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

7F11 Anti-ApcT Monoclonal Fab Light Chain, 7F11 Anti-ApcT Monoclonal Fab Heavy Chain, Uncharacterized protein MJ0609

membrane protein, transporter, antibody, Cell membrane, Membrane, Transmembrane, TRANSPORT PROTEIN, Structural Genomics, PSI-2, Protein Structure Initiative, New York Consortium on Membrane Protein Structure, NYCOMPS

MELKNKKLSLWEAVSMAVGVMIGASIFSIFGVGAKIAGRNLPETFILSGIYALLVAYSYTKLGAKIVSNAGPIAFIHKAI
GDNIITGALSILLWMSYVISIALFAKGFAGYFLPLINAPINTFNIAITEIGIVAFFTALNFFGSKAVGRAEFFIVLVKLL
ILGLFIFAGLITIHPSYVIPDLAPSAVSGMIFASAIFFLSYMGFGVITNASEHIENPKKNVPRAIFISILIVMFVYVGVA
ISAIGNLPIDELIKASENALAVAAKPFLGNLGFLLISIGALFSISSAMNATIYGGANVAYSLAKDGELPEFFERKVWFKS
TEGLYITSALGVLFALLFNMEGVASITSAVFMVIYLFVILSHYILIDEVGGRKEIVIFSFIVVLGVFLLLLYYQWITNRF
VFYGIIATFIGVLIFEIIYRKVTKRTFSNNMYVKS