Crystal structure of a cystathionine beta-synthase domain protein fused to a Zn-ribbon-like domain
Midwest Center for Structural Genomics (MCSG)
a cystathionine beta-synthase domain protein fused to a Zn-ribbon-like domain
0.2M Ammonium Chloride,20%PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 297K
AUTHORS STATE THAT THE PROTEIN WAS CRYSTALLIZED BY IN SITU PROTEOLYSIS METHOD AND THE EXACT SEQUENCE WENT INTO THE CRYSTAL WAS NOT DETERMINED. THE PROTEIN WAS MIXED WITH 100:1(W/W) WITH CHYMOTRYPSIN IMMEDIATELY PRIOR TO CRYSTALLIZATION TRIAL. THE FULL SEQUENCE BEFORE PROTEOLYSIS WAS MGSSHHHHHHSSGRENLYFQGMAQKILVEQVVKRKAIVVQPKDTVDRVAKILSRNKAG SAVVMEGDEILGVVTERDILDKVVAKGKNPKEVKVEEIMTKNPVKIEYDYDIEDVIEL MTEKGVRRVLVTKFGKPIGFVTAADILAALASHNHEEEEEEREEESEVYGICEVCGQY GALYKVYHEGRELWVCETCKDLIEGR
HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. ATOMIC B-FACTORS ARE RESIDUALS FROM TLS REFINEMENT.
Nucleotide binding protein, Metal binding protein
PF1953, APC40009, cystathionine beta-synthase domain protein, Structural Genomics, PSI-2, Protein Structure Initiative, Midwest Center for Structural Genomics, MCSG, NUCLEOTIDE BINDING PROTEIN, METAL BINDIN, Metal binding protein