Molecular mechanisms for protein-encoded inheritance.
0.2M Ammonium Sulfate, 0.1M Tris pH 8.5, 25% PEG 3350, vapor diffusion, hanging drop, temperature 298K
NNQNTF (residues 173-178) from elk prion protein
HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Authors state that the biological unit is an indefinitely long pair of sheets (a protofibril). One sheet is constructed from chain A (X,Y,Z) and unit cell translations along b cell dimension (e.g. X,Y+1,Z; X,Y-1,Z). The second sheet is constructed from crystallographic symmetry operator -x-1,y+1/2,-z-1 and unit cell translations along the b dimension (e.g. -x-1,y+3/2,-z-1). There is an additional polymorph of the biological unit (also a pair of beta sheets). One sheet is constructed from chain A (X,Y,Z) and unit cell translations along b cell dimension (e.g. X,Y+1,Z; X,Y-1,Z). The second sheet is constructed from crystallographic symmetry operator -x,y+1/2,-z-1 and unit cell translations along the b dimension (e.g. -x,y+3/2,-z-1).
amyloid-like protofibril, Cell membrane, Glycoprotein, Golgi apparatus, GPI-anchor, Lipoprotein, Membrane, Prion, PROTEIN FIBRIL