Crystal structure of alpha/beta superfamily hydrolase from Oenococcus oeni PSU-1

Midwest Center for Structural Genomics (MCSG)

SNA(MSE)KEYLTRSNISYFSIGSGTPIIFLHGLSLDKQST(CSO)LFFEPLSNVGQYQRIYLDLPG(MSE)GNSDPISP
STSDNVLETLIEAIEEIIGARRFILYGHSYGGYLAQAIAFHLKDQTLGVFLTCPVITADHSKRLTGKHINILEEDINPVE
NKEYFADFLS(MSE)NVIINNQAWHDYQNLIIPGLQKEDKTFIDQLQNNYSFTFEEKLKNINYQFPFKI(MSE)VGRNDQ
VVGYQEQLKLINHNENGEIVLLNRTGHNL(MSE)IDQREAVGFHFDLFLDELNSNN

SNAMKEYLTRSNISYFSIGSGTPIIFLHGLSLDKQSTCLFFEPLSNVGQYQRIYLDLPGMGNSDPISPSTSDNVLETLIE
AIEEIIGARRFILYGHSYGGYLAQAIAFHLKDQTLGVFLTCPVITADHSKRLTGKHINILEEDINPVENKEYFADFLSMN
VIINNQAWHDYQNLIIPGLQKEDKTFIDQLQNNYSFTFEEKLKNINYQFPFKIMVGRNDQVVGYQEQLKLINHNENGEIV
LLNRTGHNLMIDQREAVGFHFDLFLDELNSNN

SNA(MSE)KEYLTRSNISYFSIGSGTPIIFLHGLSLDKQSTCLFFEPLSNVGQYQRIYLDLPG(MSE)GNSDPISPSTSD
NVLETLIEAIEEIIGARRFILYGHSYGGYLAQAIAFHLKDQTLGVFLTCPVITADHSKRLTGKHINILEEDINPVENKEY
FADFLS(MSE)NVIINNQAWHDYQNLIIPGLQKEDKTFIDQLQNNYSFTFEEKLKNINYQFPFKI(MSE)VGRNDQVVGY
QEQLKLINHNENGEIVLLNRTGHNL(MSE)IDQREAVGFHFDLFLDELNSNN

1.6 M Ammonium sulfate, 10% Dioxane, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K

AUTHORS STATE THAT THERE IS NO ELECTRON DENSITY EVIDENCE TO SUPPORT THE RESIDUE 35 IN CHAIN D AS OXIDIZED CYSTEINE, IN CONTRAST TO ITS EVIDENT PRESENCE IN CHAINS A,B,C.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

AUTHORS STATE THAT THE BIOLOGICAL UNIT OF THIS PROTEIN IS UNKNOWN.

Alpha/beta superfamily hydrolase, PF00561, MCSG, PSI, PSI-2, Structural Genomics, Protein Structure Initiative, Midwest Center for Structural Genomics, Hydrolase

MKEYLTRSNISYFSIGSGTPIIFLHGLSLDKQSTCLFFEPLSNVGQYQRIYLDLPGMGNSDPISPSTSDNVLETLIEAIE
EIIGARRFILYGHSYGGYLAQAIAFHLKDQTLGVFLTCPVITADHSKRLTGKHINILEEDINPVENKEYFADFLSMNVII
NNQAWHDYQNLIIPGLQKEDKTFIDQLQNNYSFTFEEKLKNINYQFPFKIMVGRNDQVVGYQEQLKLINHNENGEIVLLN
RTGHNLMIDQREAVGFHFDLFLDELNSNN