Class II ligase ribozyme product-template duplex, structure 2
Structure-guided engineering of the regioselectivity of RNA ligase ribozymes.
template strand of Class II ligase A4-11
Product strand of Class II ligase A4-11, single stranded RNA with one 2'-5' linkage
0.2mM RNA duplex, 10mM MgCl2, 25% PEG 4000, 0.2M lithium sulfate, 100mM Tris 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K