Crystal Structure of MtNAS in complex with MTA and tNA

N-[(3S)-3-{[(3S)-3-amino-3-carboxypropyl]amino}-3-carboxypropyl]-L-glutamic acid

Crystallographic snapshots of iterative substrate translocations during nicotianamine synthesis in Archaea

MSCYIYWDKIKRIASRLEGMNYHFDEMDTSGVMPLLDEIEEIAHDSTIDFESAKHILDDAEMNHALSLIRKFYVNLGMKL
EMEKAQEVIESDSPWETLRSFYFYPRYLELLKNEAALGRFRRGERAVFIGGGPLPLTGILLSHVYGMRVNVVEIEPDIAE
LSRKVIEGLGVDGVNVITGDETVIDGLEFDVLMVAALAEPKRRVFRNIHRYVDTETRIIYRTYTGMRAILYAPVSDDDIT
GFRRAGVVLPSGKVNNTSVLVFKCPDKGELNSKLEGKPIPNPLLGLDSTRTGHHHHHH

22% PEG 3350, 400mM NaBr, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K

MtNAS in complex with thermonicotianamine

E81Q mutant of MtNAS in complex with L-Glutamate

E81Q mutant of MtNAS in complex with S-ADENOSYLMETHIONINE

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

thermonicotianamine, nicotianamine, BIOSYNTHETIC PROTEIN, TRANSFERASE

MSCYIYWDKIKRIASRLEGMNYHFDEMDTSGVMPLLDEIEEIAHDSTIDFESAKHILDDAEMNHALSLIRKFYVNLGMKL
EMEKAQEVIESDSPWETLRSFYFYPRYLELLKNEAALGRFRRGERAVFIGGGPLPLTGILLSHVYGMRVNVVEIEPDIAE
LSRKVIEGLGVDGVNVITGDETVIDGLEFDVLMVAALAEPKRRVFRNIHRYVDTETRIIYRTYTGMRAILYAPVSDDDIT
GFRRAGVVLPSGKVNNTSVLVFKCPD