Structure of the CFP10-ESAT6 complex from Mycobacterium tuberculosis

WXG100 protein superfamily consists of three subfamilies and exhibits an alpha-helical C-terminal conserved residue pattern.

CHIRALITY ERROR AT CB CENTER OF THR B 75

CFP-10, 10 kDa culture filtrate antigen cfp10, Secreted antigenic protein MTSA-10

AMAEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELDEISTNIRQA
GVQYSRADEEQQQALSSQMGF

TEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQNLARTISEAGQ
AMASTEGNVTGMFA

The construct contained the entire protein encoding region of the CFP10-ESAT6 operon including its intergenic region

33% (v/v) PEG 600, 200mM zinc acetate, 100mM imidazol pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Complex, Operon structure, Four-helical-bundle, Coiled-coil, WXG-motif, Mycobacterium tuberculosis, secreted, secretion system, adaptor protein, proposed virulent factor, VIRAL PROTEIN

MAEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELDEISTNIRQAG
VQYSRADEEQQQALSSQMGF