Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)

Structure and cleavage specificity of the chymotrypsin-like serine protease (3CLSP/nsp4) of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV).

GPLGSPEFPGAFRTQKPSLNTVNVVGSSMGSGGVFTIDGKIKCVTAAHVLTGNSARVSGVGFNQMLDFDVKGDFAIADCP
NWQGVAPKAQFCEDGWTGRAYWLTSSGVEPGVIGNGFAFCFTACGDSGSPVITEAGELVGVHTGSNKQGGGIVTRPSGQF
CNVKPIKLSELSEFFAGPKVPLGDVKIGSHIIKDTCEVPSDLCALLAAKPELE

Porcine respiratory and reproductive syndrome virus

0.1M sodium citrate pH 5.3, 0.7M ammonium phosphate monobasic, VAPOR DIFFUSION, HANGING DROP, temperature 291K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

chymotrypsin-like, N-terminal beta-barrels, C-terminal alpha-beta extra domain, canonical catalytic triad, HYDROLASE

GAFRTQKPSLNTVNVVGSSMGSGGVFTIDGKIKCVTAAHVLTGNSARVSGVGFNQMLDFDVKGDFAIADCPNWQGVAPKA
QFCEDGWTGRAYWLTSSGVEPGVIGNGFAFCFTACGDSGSPVITEAGELVGVHTGSNKQGGGIVTRPSGQFCNVKPIKLS
ELSEFFAGPKVPLGDVKIGSHIIKDTCEVPSDLCALLAAKPELE