Crystal structure of a complex between integrin beta4 and plectin

Structural basis of the interaction between integrin alpha6beta4 and plectin at the hemidesmosomes

Fibronectin type-III, residues 1126-1370

Plectin 1C, PLTN, PCN, Hemidesmosomal protein 1, HD1, Plectin-6, Plectin-11

GSHMSGEDAEVRAVSEDVSNGSSGSPSPGDTLPWNLGKTQRSRRSGGGAGSNGSVLDPAERAVIRIADERDRVQKKTFTK
WVNKHLIKAQRHISDLYEDLRDGHNLISLLEVLSGDSLPREKGRMRFHKLQNVQIALDYLRHRQVKLVNIRNDDIADGNP
KLTLGLIWTIILHFQISDIQVSGQSEDMTAKEKLLLWSQRMVEGYQGLRCDNFTSSWRDGRLFNAIIHRHKPLLIDMNKV
YRQTNLENLDQAFSVAERDLGVTRLLDPEDVDVPQPDEKSIITYVSSLYDAMPRVP

GSHDLGAPQNPNAKAAGSRKIHFNWLPPSGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQ
GEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNDDNRPIGPMKKVLVDNPKNRM
LLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYDSFLMYSDDVLRSPSGSQR
PSVSDDTE

0.1M Sodium Acetate, 0.2M CaCl2, 10% PEG 6000, pH 5.8, VAPOR DIFFUSION, temperature 298K

Structure of a similar fragment of integrin beta4 as the one used in the current structure, but lacking the connecting segment

Structure of the plectin fragment used in the current structure, but in the absence of integrin beta4

STRUCTURE AT 1.75A OF THE FRAGMENT OF INTEGRIN BETA4 IN THE ABSENCE OF PLECTIN

STRUCTURE AT 2.04A OF A SIMILAR FRAGMENT OF INTEGRIN BETA4 IN THE ABSENCE OF PLECTIN

THE SEQUENCES OF PLECTIN-1, INTEGRIN BETA-4 ARE BASED ON REFERENCE 2 IN THE DATABASE, PLEC1_HUMAN, ITB4_HUMAN, RESPECTIVELY.

The biological unit is the beta4-plectin heterodimer. The asymmetric unit contains two copies of the beta4-plectin heterodimer and a beta4 molecule not bound to plectin.

INTEGRIN, PLAKIN, HEMIDESMOSOME, CELL ADHESION, EPIDERMOLYSIS BULLOSA, Actin-binding, Alternative splicing, Coiled coil, Cytoplasm, Cytoskeleton, Disease mutation, Phosphoprotein, Structural protein, Glycoprotein, Membrane, Polymorphism, Receptor, Transmembrane, STRUCTURAL PROTEIN-CELL ADHESION COMPLEX

chain A and resseq 60:62 and (name CA or name C or name N or name O or name CB)

chain B and resseq 60:62 and (name CA or name C or name N or name O or name CB)

chain A and (resseq 65:144 or resseq 146:179 ) and not ((resseq 69 or resseq 73 or resseq 95 or resseq 117 or resseq 158) and sidechain)

chain B and (resseq 65:144 or resseq 146:179 ) and not ((resseq 69 or resseq 73 or resseq 95 or resseq 117 or resseq 158) and sidechain)

chain D and (resseq 1127:1138 or resseq 1142:1157 or resseq 1159:1162 or resseq 1172:1192 or resseq 1194:1216 or (resseq 1158 and backbone) or (resseq 1193 and backbone) )

chain C and (resseq 1127:1138 or resseq 1142:1157 or resseq 1159:1162 or resseq 1172:1192 or resseq 1194:1216 or (resseq 1158 and backbone) or (resseq 1193 and backbone) )

chain E and (resseq 1127:1138 or resseq 1142:1157 or resseq 1159:1162 or resseq 1172:1192 or resseq 1194:1216 or (resseq 1158 and backbone) or (resseq 1193 and backbone) )

chain C and (resseq 1217:1224 or resseq 1226:1261 or resseq 1271:1273 or resseq 1283:1286 or resseq 1288:1315 or (resseq 1225 and backbone)) and not ((resseq 1225 or resseq 1237 or resseq 1301) and sidechain)

chain D and (resseq 1217:1224 or resseq 1226:1261 or resseq 1271:1273 or resseq 1283:1286 or resseq 1288:1315 or (resseq 1225 and backbone)) and not ((resseq 1225 or resseq 1237 or resseq 1301) and sidechain)

chain E and (resseq 1217:1224 or resseq 1226:1261 or resseq 1271:1273 or resseq 1283:1286 or resseq 1288:1315 or (resseq 1225 and backbone)) and not ((resseq 1225 or resseq 1237 or resseq 1301) and sidechain)

MSGEDAEVRAVSEDVSNGSSGSPSPGDTLPWNLGKTQRSRRSGGGAGSNGSVLDPAERAVIRIADERDRVQKKTFTKWVN
KHLIKAQRHISDLYEDLRDGHNLISLLEVLSGDSLPREKGRMRFHKLQNVQIALDYLRHRQVKLVNIRNDDIADGNPKLT
LGLIWTIILHFQISDIQVSGQSEDMTAKEKLLLWSQRMVEGYQGLRCDNFTSSWRDGRLFNAIIHRHKPLLIDMNKVYRQ
TNLENLDQAFSVAERDLGVTRLLDPEDVDVPQPDEKSIITYVSSLYDAMPRVP

DLGAPQNPNAKAAGSRKIHFNWLPPSGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEG
PYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNDDNRPIGPMKKVLVDNPKNRMLLI
ENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYDSFLMYSDDVLRSPSGSQRPSV
SDDTE