Crystal structure of Toxoplasma gondii micronemal protein 1 bound to 2F-3'SiaLacNAc

2-FLUORO-2-DEOXY-BETA-D-GALACTOPYRANOSE; 2-deoxy-2-fluoro-beta-D-galactose; 2-deoxy-2-fluoro-D-galactose; 2-deoxy-2-fluoro-galactose

N-acetylneuraminic acid; sialic acid; alpha-sialic acid; O-SIALIC ACID

Detailed insights from microarray and crystallographic studies into carbohydrate recognition by microneme protein 1 (MIC1) of Toxoplasma gondii.

Atomic resolution insight into host cell recognition by Toxoplasma gondii.

N-acetyl-alpha-neuraminic acid-(2-3)-2-deoxy-2-fluoro-beta-D-galactopyranose

VGPEAYGEASHSHSPASGRYIQQ(MSE)LDQRCQEIAAELCQSGLRK(MSE)CVPSSRIVARNAVGITHQNTLQWRCFDT
ASLLESNQENNGVNCVDDCGHTIPCPGGVHRQNSNHATRHEILSKLVEEGVQRFCSPYQASANKYCNDKFPGTIARRSKG
FGNNVEVAWRCYEKASLLYSVYAECASNCGTTWYCPGGRRGTSTELDKRHYTEEEGIRQAIGSVDSPCSEVEVCLPKDEN
PPLCLDESGQISRT

VGPEAYGEASHSHSPASGRYIQQMLDQRCQEIAAELCQSGLRKMCVPSSRIVARNAVGITHQNTLQWRCFDTASLLESNQ
ENNGVNCVDDCGHTIPCPGGVHRQNSNHATRHEILSKLVEEGVQRFCSPYQASANKYCNDKFPGTIARRSKGFGNNVEVA
WRCYEKASLLYSVYAECASNCGTTWYCPGGRRGTSTELDKRHYTEEEGIRQAIGSVDSPCSEVEVCLPKDENPPLCLDES
GQISRT

3.6M Ammonium acetate, 100mM Bis/tris-propane, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K

CRYSTAL STRUCTURE OF TOXOPLASMA GONDII MICRONEMAL PROTEIN 1

CRYSTAL STRUCTURE OF TOXOPLASMA GONDII MICRONEMAL PROTEIN 1 BOUND TO 3'-SIALYL-N-ACETYLLACTOSAMINE

CRYSTAL STRUCTURE OF TOXOPLASMA GONDII MICRONEMAL PROTEIN 1 BOUND TO 6'-SIALYL-N-ACETYLLACTOSAMINE

CRYSTAL STRUCTURE OF TOXOPLASMA GONDII MICRONEMAL PROTEIN 1 BOUND TO 3'SiaLacNAc1-3'

CRYSTAL STRUCTURE OF TOXOPLASMA GONDII MICRONEMAL PROTEIN 1 BOUND TO 2'F-3'SiaLacNAc1-3

THE NAG RESIDUE IS DISORDERED IN 2F-3'SIALACNAC AND NOT VISIBLE IN THE ELECTRON DENSITY, THEREFORE IS MISSING IN THE FINAL STRUCTURE.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

cell adhesion, micronemal protein, carbohydrate, fluorine, toxoplasma gondii, Cytoplasmic vesicle, Lectin, Virulence