Structures of the multicomponent Rieske non-heme iron toluene 2,3-dioxygenase enzyme system

Purification, crystallization and preliminary X-ray diffraction studies of the three components of the Toluene 2,3-dioxygenase enzyme system

Benzene 1,2-dioxygenase P1 subunit, Toluene 2,3-dioxygenase subunit alpha

Benzene 1,2-dioxygenase P2 subunit, Toluene 2,3-dioxygenase subunit beta

MNQTDTSPIRLRRSWNTSEIEALFDEHAGRIDPRIYTDEDLYQLELERVFARSWLLLGHETQIRKPGDYITTYMGEDPVV
VVRQKDASIAVFLNQCRHRGMRICRADAGNAKAFTCSYHGWAYDTAGNLVNVPYEAESFACLNKKEWSPLKARVETYKGL
IFANWDENAVDLDTYLGEAKFYMDHMLDRTEAGTEAIPGVQKWVIPCNWKFAAEQFCSDMYHAGTTSHLSGILAGLPEDL
EMADLAPPTVGKQYRASWGGHGSGFYVGDPNLMLAIMGPKVTSYWTEGPASEKAAERLGSVERGSKLMVEHMTVFPTCSF
LPGINTVRTWHPRGPNEVEVWAFTVVDADAPDDIKEEFRRQTLRTFSAGGVFEQDDGENWVEIQHILRGHKARSRPFNAE
MSMDQTVDNDPVYPGRISNNVYSEEAARGLYAHWLRMMTSPDWDALKATR

MIDSANRADVFLRKPAPVAPELQHEVEQFYYWEAKLLNDRRFEEWFALLAEDIHYFMPIRTTRIMRDSRLEYSGSREYAH
FDDDATMMKGRLRKITSDVSWSENPASRTRHLVSNVMIVGAEAEGEYEISSAFIVYRNRLERQLDIFAGERRDTLRRNTS
EAGFEIVNRTILIDQSTILANNLSFFF

40%(w/v) polyethylene glycol 600, 0.1 M sodium citrate pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 286K

Structure of Toluene 2,3-Dioxygenase Reductase component

Structure of Toluene 2,3-Dioxygenase Ferredoxin component

Structure of Toluene 2,3-Dioxygenase Oxygenase component

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Rieske iron sulfur binding protein, apo-structure, 2Fe-2S, Aromatic hydrocarbons catabolism, Dioxygenase, Iron, Iron-sulfur, Metal-binding, NAD, Oxidoreductase