The crystal structure of putative zinc protease beta-subunit from Thermus thermophilus HB8

Crystal structure of TTHA1264, a putative M16-family zinc peptidase from Thermus thermophilus HB8 that is homologous to the beta subunit of mitochondrial processing peptidase.

(MSE)FREAELRNGLRVIAEVVPGARSVALGYFVKTGARDETKEESGVSHFLEH(MSE)VFKGPED(MSE)DALAVNRAF
DR(MSE)GAQYNAFTSEEATVYYGAVLPEFAYDLLGLFAKLLRPALREEDFQTEKLVILEEIARYQDRPGF(MSE)AYEW
ARARFFQGHPLGNSVLGTRESITALTREG(MSE)AAYHRRRYLPKN(MSE)VLAATGRVDFDRLLAEAERLTEAWPEGEA
ERAYPPLTPAFGVEERPYEKARALYLVALFPGVAYQEEARFPGQVLAHLLGEEGSGRLHFALVDKGLAEVASFGLEEADR
AGTFHAYVQADPARKGEVLAVLQEELDRLGREGVGEEEVERAKTPLATGLVFAGETP(MSE)QRLFHLG(MSE)EYLYTG
RYLSLEEVKARVQRVTSREVNALLERGFLEKGLYYLVLPHGA

MFREAELRNGLRVIAEVVPGARSVALGYFVKTGARDETKEESGVSHFLEHMVFKGPEDMDALAVNRAFDRMGAQYNAFTS
EEATVYYGAVLPEFAYDLLGLFAKLLRPALREEDFQTEKLVILEEIARYQDRPGFMAYEWARARFFQGHPLGNSVLGTRE
SITALTREGMAAYHRRRYLPKNMVLAATGRVDFDRLLAEAERLTEAWPEGEAERAYPPLTPAFGVEERPYEKARALYLVA
LFPGVAYQEEARFPGQVLAHLLGEEGSGRLHFALVDKGLAEVASFGLEEADRAGTFHAYVQADPARKGEVLAVLQEELDR
LGREGVGEEEVERAKTPLATGLVFAGETPMQRLFHLGMEYLYTGRYLSLEEVKARVQRVTSREVNALLERGFLEKGLYYL
VLPHGA

0.15M DL-MALIC ACID, 22% PEG 3350, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

two similar domains of beta(2)-alpha(2)-beta(2)-alpha(5)-beta structure, Protease, HYDROLASE