Crystal structure of Amino Transferase (RER070207001803) from Eubacterium rectale at 2.10 A resolution

Joint Center for Structural Genomics (JCSG)

Flat collimating mirror, toroid focusing mirror

G(MSE)VVNES(MSE)YQLGSVRSAIRELFEYGKKRAAIVGKENVYDFSIGNPSIPAPQIVNDTIKELVTDYDSVALHGY
TSAQGDVETRAAIAEFLNNTHGTHFNADNLY(MSE)T(MSE)GAAASLSICFRALTSDAYDEFITIAPYFPEYKVFVNAA
GARLVEVPADTEHFQIDFDALEERINAHTRGVIINSPNNPSGTVYSEETIKKLSDLLEKKSKEIGRPIFIIADEPYREIV
YDGIKVPFVTKYYDNTLVCYSYSKSLSLPGERIGYVLVPDEVYDKAELYAAVCGAGRALGYVCAPSLFQK(MSE)IVKCQ
GATGDINAYKENRDLLYEGLTRIGYHCFKPDGAFY(MSE)FVKALEDDSNAFCEKAKEEDVLIVAADGFGCPGWVRISYC
VDRE(MSE)IKHS(MSE)PAFEKIYKKYNK

GMVVNESMYQLGSVRSAIRELFEYGKKRAAIVGKENVYDFSIGNPSIPAPQIVNDTIKELVTDYDSVALHGYTSAQGDVE
TRAAIAEFLNNTHGTHFNADNLYMTMGAAASLSICFRALTSDAYDEFITIAPYFPEYKVFVNAAGARLVEVPADTEHFQI
DFDALEERINAHTRGVIINSPNNPSGTVYSEETIKKLSDLLEKKSKEIGRPIFIIADEPYREIVYDGIKVPFVTKYYDNT
LVCYSYSKSLSLPGERIGYVLVPDEVYDKAELYAAVCGAGRALGYVCAPSLFQKMIVKCQGATGDINAYKENRDLLYEGL
TRIGYHCFKPDGAFYMFVKALEDDSNAFCEKAKEEDVLIVAADGFGCPGWVRISYCVDREMIKHSMPAFEKIYKKYNK

1.0000M LiCl, 20.0000% PEG-6000, 0.1M HEPES pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K

THE CONSTRUCT WAS EXPRESSED WITH A PURIFICATION TAG MGSDKIHHHHHHENLYFQG. THE TAG WAS REMOVED WITH TEV PROTEASE LEAVING ONLY A GLYCINE (0) FOLLOWED BY THE TARGET SEQUENCE.

1. HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
2. ATOM RECORDS CONTAIN RESIDUAL B FACTORS ONLY.
3. A MET-INHIBITION PROTOCOL WAS USED FOR SELENOMETHIONINE
INCORPORATION DURING PROTEIN EXPRESSION. THE OCCUPANCY
OF THE SE ATOMS IN THE MSE RESIDUES WAS REDUCED TO 0.75
FOR THE REDUCED SCATTERING POWER DUE TO PARTIAL S-MET INCORPORATION.
4. EDO MOLECULES FROM THE CRYO SOLUTION ARE MODELED.
5. LIGAND MOLECULE PLP IS MODELED ON THE BASIS OF DENSITY AND
HOMOLOGOUS STRUCTURES 1O4S, 1XI9.
6. RAMACHANDRAN OUTLIER RESIDUES 217 ARE WELL SUPPORTED BY DENSITY.

AUTHORS STATE THAT CRYSTAL PACKING ANALYSIS SUGGESTS THE ASSIGNMENT OF A DIMER AS THE SIGNIFICANT OLIGOMERIZATION STATE.

RER070207001803, Amino Transferase, Structural Genomics, Joint Center for Structural Genomics, JCSG, Protein Structure Initiative, PSI-2, Aminotransferase class I and II, TRANSFERASE