CRYSTAL STRUCTURE OF A PHENYLACETATE-COA OXYGENASE SUBUNIT PAAB (REUT_A2307) FROM RALSTONIA EUTROPHA JMP134 AT 2.65 A RESOLUTION
Joint Center for Structural Genomics (JCSG)
Crystal structure of phenylacetate-CoA oxygenase subunit PaaB (YP_297411.1) from RALSTONIA EUTROPHA JMP134 at 2.65 A resolution
Flat collimating mirror, toroid focusing mirror
phenylacetate-CoA oxygenase subunit PaaB
G(MSE)TQKEWPLWEVFVRSKQGLEHKHCGSLHATDAQQALH(MSE)ARDVYTRRQEGVSIWVVPSTAITASAPEEKPEL FDP(MSE)ADKIYRHPTFYQLPDEVNH(MSE)
GMTQKEWPLWEVFVRSKQGLEHKHCGSLHATDAQQALHMARDVYTRRQEGVSIWVVPSTAITASAPEEKPELFDPMADKI YRHPTFYQLPDEVNHM
0.2000M KThioCyanate, 20.0000% PEG-3350, No Buffer pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K
THE CONSTRUCT WAS EXPRESSED WITH A PURIFICATION TAG MGSDKIHHHHHHENLYFQG. THE TAG WAS REMOVED WITH TEV PROTEASE LEAVING ONLY A GLYCINE (0) FOLLOWED BY THE TARGET SEQUENCE.
(1). HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. (2). A MET-INHIBITION PROTOCOL WAS USED FOR SELENOMETHIONINE INCORPORATION DURING PROTEIN EXPRESSION. THE OCCUPANCY OF THE SE ATOMS IN THE MSE RESIDUES WAS REDUCED TO 0.75 TO ACCOUNT FOR THE REDUCED SCATTERING POWER DUE TO PARTIAL S-MET INCORPORATION. (3). ATOM RECORD CONTAINS RESIDUAL B FACTORS ONLY (4). UNEXPLAINED ELECTRON DENSITIES NEAR RESIDUE 6 IN B CHAIN WERE NOT MODELED. (5). THIOCYANATE (SCN) IONS FROM CRYO SOLUTION WERE MODELED. (6). THE RESIDUES 66-95 IN A AND B CHAINS WERE NOT VISIBLE IN THE ELECTRON DENSITY MAPS AND THEY WERE NOT MODELED.
PHENYLACETATE-COA OXYGENASE SUBUNIT PAAB, STRUCTURAL GENOMICS, JOINT CENTER FOR STRUCTURAL GENOMICS, JCSG, PROTEIN STRUCTURE INITIATIVE, PSI-2, OXIDOREDUCTASE
MTQKEWPLWEVFVRSKQGLEHKHCGSLHATDAQQALHMARDVYTRRQEGVSIWVVPSTAITASAPEEKPELFDPMADKIY RHPTFYQLPDEVNHM