Crystal structure of human CD38 extracellular domain, ribose-5'-phosphate intermediate/GTP complex

2-amino-9-{5-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-ribofuranosyl}-9H-purin-6-yl 5-O-phosphono-beta-D-ribofuranoside

Covalent and Noncovalent Intermediates of an NAD Utilizing Enzyme, Human CD38.

Cyclic ADP-ribose hydrolase 1, cADPr hydrolase 1, T10, CD38 antigen

KREAEARWRQTWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCDITEEDYQPLMKLGTQTVP
CNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFDTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAE
AACDVVHVMLDGSRSKIFDKDSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNI
YRPDKFLQCVKNPEDSSCTSEI

100 mM MES pH 6.0, 15% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Human CD38 extracellular domain, ara-2-deoxy-2-fluoro-ribose-5'-phosphate complex

Human CD38 extracellular domain, ara-2-deoxy-2-fluoro-ribose-5'-phosphate/nicotinamide complex

Human CD38 extracellular domain, GTP complex

Human CD38 extracellular domain E226Q mutant, NMN complex

Human CD38 extracellular domain, NMN complex

AUTHORS STATE THAT THE LIGAND [N] IN THIS ENTRY IS A REACTION INTERMEDIATE, WITH THE (C1') ATOM HAVING SP2 HYBRIDIZATION (C1'H), NOT THE SP3 HYBRIDIZATION (C1'H2). AUTHORS ALSO STATE THAT THE LIGAND RGT THAT IS PRESENT IN THIS ENTRY IS ALSO AN UNSTABLE INTERMEDIATE AND THEREFORE THE DISTORTED LINKAGE BETWEEN RIBOSE-PHOSPHATE AND GTP MOIETIES REFLECTS THE CHEMISTRY OF THE REACTION.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Noncovalent intermediate, GTP complex, R5P-GTP adduct, BETA SHEETS, ALPHA BUNDLE, Alternative splicing, Diabetes mellitus, Glycoprotein, Hydrolase, Membrane, NAD, Polymorphism, Receptor, Signal-anchor, Transmembrane

RWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITEEDYQPLMKLGTQTVPCNKILL
WSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVV
HVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKF
LQCVKNPEDSSCTSEI